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《Chinexe Journal of Pediatrics》 2002-01
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Experimental peripheral neuropathy induced by serum and peripheral blood mononuclear cells immunized with Campylobacter jejuni in rats

ZHANG Jiemin, CAI Fangcheng. Department of Neurology, Chongqing Children′s Hospital, Chongqing University of Medical Sciences, Chongqing 400014, China  
Objective Resently, it had been found that Guillain-Barre syndrome (GBS) is associated with Compylobacter jejuni (CJ), as anti-CJ antibodies have been found in the serum of these patients. Nevertheless, whether the antibodies are related with the pathologic changes in peripheral nerves need to be elucidated. In the study, to clarify the association of CJ serotypes with GBS and to observe the different effects of humoral and cellular immune on peripheral nerve pathologic changes, the authors transferred the serum and peripheral blood mononuclear cells (PBMC) from CJ-immunized rats into naive rats perineurally and compared the characteristic pathologic changes between different groups. Method Two groups were established including the group of injection with serum and the group with PBMC perineurally. Each group subdivided again into CJ-Pen19 and Pen43 groups, respectively. The sciatic nerves were harvested at the 7th day after the injection and were taken the pathologic examination. Results (1) In the groups of CJ-immunized serum injection: severe pathologic change showed in 100% of sciatic nerves and the incidence of pathologic fibers was up to 87%, which included 75% of axon degeneration and 12% of demyelination in CJ-Pen19-subgroup. While in Pen43-subgroup, only the moderate changes happened in 50% of sciatic nerves and the incidence of pathologic fibers was only 27.1%, which included 22.8% of axon degeneration and 4.3% of demyelination (χ 2=743.8, P0.01). (2) Comparing the groups of CJ-Pen19 and Pen43 with injection of PBMCs: the PBMC stimulated with associated-CJ antigen resulted in 30% and 20% pathologic changes in sciatic nerves (χ 2=0.27, P0.05),respectively. The incidences of the axon degeneration were 8.0% and 8.2% (χ 2=0.04, P0.05) and, the demyelinations were 9.0% and 11.8% (χ 2=5.84, P0.05); the PBMC stimulated with PHA resulted in 40% and 30% pathologic changes in sciatic nerves (χ 2=0.22, P0.05), the incidences of axon degeneration were 21.0% and 21.3% (χ 2=0.02, P0.05) and, the demyelinations were 38.3% and 44.7% (χ 2=6.33, P0.05), respectively. Conclusion The incidence of pathologic changes in peripheral nerves induced by CJ was related closely with the CJ serotypes. The CJ immuned-serum caused axon degeneration seriously, while the PBMC resulted in both axon degeneration and demyelination of the sciatic nerve at the same time.
【CateGory Index】: R745.43
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