Expression of E-selectin in cerebrovascular endothelium in a brain inflammation model
LING Yaping *, XIAO Desheng. *Department of Pediatrics, The Affiliated Hospital of Zhenjiang Medical College, Zhenjiang 212001, China
Objective This study was aimed to explore the changes of expression of E-selectin on the cerebrovascular endothelium in the central nervous system with the inflammation and to evaluate the roles of dexamethasone. Methods Fifty-four mice were randomly divided into the following groups: endotoxin (20 μg/20 μl of saline) group, endotoxin (20 μg/20 μl of saline) plus dexamethasone [i.p.,1 mg/(kg·12h)] group, saline group and controls. The information of E-selectin expression in the cerebrovascular endothelium was obtained by observing brain tissue sections staining with the immunohistochemical method. HE staining on brain tissue sections was also performed to observe the profile of the brain inflammation reaction induced by endotoxin. Results A weak expression of E-selectin was found in the control group [(13.7±2.7)%]. The administration of saline [(13.5±5.6)%] did not affect E-selectin expression. As compared to the saline group, E-selectin expression in the endotoxin group was significantly enhanced at day 1 [(31.8±10.5)%, t = 3.77, P0.01] and day 2 [(26.8±9.6)%, t=2.94, P0.05] with the inflammation reactions including the brain edema, intravascular adhesion, extravascular infiltration of lymphocytes and neutrocytes, and enlarged surrounding interstitium of vascules. After 3 days of the endotoxin injection, the E-selcetin expression [(15.7±9.9)%, t=0.47, P0.05] was similar to that of the saline group, and the inflammation reaction was mildened. The E-selectin expression was progressively declined during the first 3 days of the injection (F=4.08,P0.05). As compared to the endotoxin group, the E-selectin expression in the endotoxin plus dexamethasone group was significantly inhibited at day 1 [(13.0±12.4)%, t=2.16, P0.05] and day 2 [(8.8±6.0)%, t=2.64, P0.05], and the inflammation reactions were significantly blocked by the injection of dexamethasone. No differences in the expression of E-selectin were found among the endotoxin plus dexamethasone group (at day 1, 2 and 3) , the saline group and controls (all P0.05). Conclusion The over-expression of E-selectin on vessel endothelial cells of brain may be involved in the process of inflammation of the central nervous system. Dexamethasone may inhibit the brain inflammatory reaction, possibly through its inhibition of the E-selectin expression on vessel endothelial cells.
【Fund】： 江苏省人事厅科研基金;; 镇江市科委研究基金;; 镇江医学院博士研究基金资助
【CateGory Index】： R741.02
【CateGory Index】： R741.02