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STUDIES ON COUNTERACTING CHROMATOGRAPHIC ELECTROPHORESIS AND ITS UTILIZATION FOR PROTEIN PURIFICATION

Gao Ruichang Jin Manrong and Wang Shichang Chemical Engineering Research Centre, Tianjin Univercity, Tianjin 300072, China Department of Pharmacy, Tianjin Second Medical College, Tianjin. To whom correspondence should be addressed.  
A counteracting chromatographic electrophoresis (CAGE) separation device has been successfully built and tested. Experiments are performed with protein mixtures of BSA, Hg, Mg and Cyto-c to study the separation performances, concentration profiles, start-up methodology as well as influences of pH and ionic strength on the separation process. Mathematical models based on the fluid dynamics of the porous medium are proposed. Experiments indicate that the CACE process has a good commercial potential in protein engineering and fine chemicals purification.
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