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《中国化学工程学报(英文版)》 1996-01
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MEASUREMENT AND CORRELATION OF PARTITION COEFFICIENTS OF UROKINASE IN AQUEOUS TWO PHASE PEG-POTASSIUM PHOSPHATE SYSTEM

Zhu Ziqiang Li Mian Mei Lehe Han Zhaoxiong Department of Chemical Engineering, Zhejiang University, Hangzhou 310027, China To whom correspondence should be addressed.  
Partition coefficients of Urokinase (UK) were measured in aqueous two-phase systems containing polyethylene glycol and potassium phosphate at 273.2K. Based on the Diamond-Hsu model, a modified expression was obtained for the correlation of enzyme partitioning in the above mentioned systems. Utilizing a modified form, the partitioning data of UK and heteroprotein were correlated. The results show that the modified model is simple, gives good precision, and will facilitate engineering scale-up of aqueous two phase systems for certain proteins purification.
【Fund】: Supported by National Natural Science Foundation of China
【CateGory Index】: TQ016
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