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《中国化学工程学报(英文版)》 2008-06
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New Development of Reverse Micelles and Applications in Protein Separation and Refolding

LIU Yang,DONG Xiaoyanand SUN Yan Department of Biochemical Engineering,School of Chemical Engineering and Technology,Tianjin University, Tianjin 300072,China  
Reverse micelles bring mild and effective microenvironments in organic solvent that contain biomolecules,which have attracted immense attention for application in the isolation of proteins,protein refolding,and enzymatic reaction.In this review,the application of reverse micelles for protein separation and refolding has been briefly summarized and various reverse micellar systems composed of different surfactants,including ionic,nonionic,mixed,and affinity-based reverse micelles,have been highlighted.It illustrates especially the potential appli- cation of the novel affinity-based reverse micelles consisting of biocompatible surfactant coupled with affinity ligands.Moreover,the importance to develop universal affinity-based reverse micelles for protein separation and refolding in the downstream processing of biotechnology has been pointed out.
【Fund】: Supported by the National Natural Science Foundation of China(20676098)
【CateGory Index】: O629.73
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1 ZHAO Xi-hong~(1,2),HE Xiao-wei~(1,2,*),LI Wen-mei~(1,2),YANG Lian-sheng~1, WANG Ji-hua~(1,2),PENG Yun-ping~(1,2),LIU Xiao-yun~(1,2) (1.College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China; 2.Guangzhou Wondfo Biotech CO.,LTD.,Guangzhou 510640,China);Development of in-vitro refolding of inclusion body proteins in E.coli[J];Science and Technology of Food Industry;2010-06
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1 HUANG Ya-Yu1, TONG Wei2, GUO Li-An3, GENG Xin-Du4 1Department of Hematology, 2Department of Medical Affairs, Xijing Hospital, 3Departmant of Chemistry, School of Pharmacy, Fourth Military Medical University, Xi'an 710033, China, 4Departmant of Chemistry, North-West University, Xi'an 710069, China;Refolding of reduced/denatured ribonuclease by high performance liquid chromatography[J];Journal of The Fourth Military Medical University;2005-15
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