Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《中国化学工程学报(英文版)》 2008-06
Add to Favorite Get Latest Update

New Development of Reverse Micelles and Applications in Protein Separation and Refolding

LIU Yang,DONG Xiaoyanand SUN Yan Department of Biochemical Engineering,School of Chemical Engineering and Technology,Tianjin University, Tianjin 300072,China  
Reverse micelles bring mild and effective microenvironments in organic solvent that contain biomolecules,which have attracted immense attention for application in the isolation of proteins,protein refolding,and enzymatic reaction.In this review,the application of reverse micelles for protein separation and refolding has been briefly summarized and various reverse micellar systems composed of different surfactants,including ionic,nonionic,mixed,and affinity-based reverse micelles,have been highlighted.It illustrates especially the potential appli- cation of the novel affinity-based reverse micelles consisting of biocompatible surfactant coupled with affinity ligands.Moreover,the importance to develop universal affinity-based reverse micelles for protein separation and refolding in the downstream processing of biotechnology has been pointed out.
【Fund】: Supported by the National Natural Science Foundation of China(20676098)
【CateGory Index】: O629.73
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 ZHAO Xi-hong~(1,2),HE Xiao-wei~(1,2,*),LI Wen-mei~(1,2),YANG Lian-sheng~1, WANG Ji-hua~(1,2),PENG Yun-ping~(1,2),LIU Xiao-yun~(1,2) (1.College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China; 2.Guangzhou Wondfo Biotech CO.,LTD.,Guangzhou 510640,China);Development of in-vitro refolding of inclusion body proteins in E.coli[J];Science and Technology of Food Industry;2010-06
2 LIU Jin-Yan HAN Wai-Hui ZHANG Yan YAO Chao-Huai(College of Chemistry and Chemical Engineering,Inner Mongolia University of Science and Technology,Baotou 014010,Inner Mongolia,P.R.China);Studies on the Conductivity of Mixed Reverse Micelles of Anionic and Non-Ionic Surfactants[J];Acta Physico-Chimica Sinica;2010-06
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 LI Kai,LI Cheng-fu,LI Jia-you,LIU Qian,JIAO Qing-cai(The State Key Laboratory of Pharmaceutical Biotechnology,School of Life Science,Nanjing University,Nanjing 210093,Jiangsu,China);Reversed Micellar Extraction of Arginine Deiminase[J];Fine Chemicals;2008-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HUANG Ya-Yu1, TONG Wei2, GUO Li-An3, GENG Xin-Du4 1Department of Hematology, 2Department of Medical Affairs, Xijing Hospital, 3Departmant of Chemistry, School of Pharmacy, Fourth Military Medical University, Xi'an 710033, China, 4Departmant of Chemistry, North-West University, Xi'an 710069, China;Refolding of reduced/denatured ribonuclease by high performance liquid chromatography[J];Journal of The Fourth Military Medical University;2005-15
2 Qin Ming Zhang Chao Zhan Wang* Jiang Feng Liu Li Li Wang Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education,Institute of Modern Separation Science,Key Laboratory of Separation Science in Shanxi Province,Department of Chemistry,Northwest University,Xi'an 710069,China;A novel protein refolding method integrating ion exchange chromatography with artificial molecular chaperone[J];中国化学快报(英文版);2008-05
3 BAI Quan *, KONG Yu, GENG Xin Du(Institute of Modern Separation Science, Key Lab of Separation Science in Shaanxi Province, Northwest University, Xiaan 710069, China);Studies on the Refolding of Reduced-Denaturated Insulin with High Performance Hydrophobic Interaction Chromatography[J];Chemical Research In Chinese Universities;2002-08
4 Gu Zhenyu and Su Zhiguo (State Key Laboratory of Biochemical Engineering, Instigate of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080);CHROMATOGRAPHIC PROTEIN REFOLDING[J];Journal of Chemical Industry and Engineering(China);2000-S1
5 WANG Jun, LU Diannan, LIN Ying, ZHOU Rui and LIU Zheng(Department of Chemical Engineering, Tsinghua university, Beijing 100084,China);SURFACTANT ASSISTED LYSOZYME REFOLDING:CHARACTERIZATION OF STRUCTURE OF SURFACTANT-DENATURED LYSOZYME COMPLEX[J];Journal of Chemical Industry and Engineering(China);2004-08
6 LIU, Hong-Ni WANG, Yan GONG, Bo-Lin GENG, Xin-Du* (Institute of Modern Separation Science, Key Laboratory of Modern Separation Science of Shaanxi Province, Xi'an 710069);Study on Refolding of Urea-Reduced/Denatured Lysozyme by High-Performance Weak-cation Exchange Chromatography[J];Acta Chimica Sinica;2005-07
7 ZHANG You-shang(Shanghai Institutes for Biological Sciences,CAS,Shanghai 200031,China);The Production of Insulin:Retrospect and Prospect[J];Food and Drug;2008-01
8 ZHAO Xi-hong1,HE Xiao-wei1,YANG Lian-sheng1,WANG Ji-hua2,PENG Yun-ping2(1.College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China;(2.Guangzhou Wondfo Biotech. Co.,Ltd.,Guangzhou 510640,China);Development in protein extraction by reverse micelles[J];Science and Technology of Food Industry;2009-02
9 LI Min~1,YANG Xiao-hui~1,GENG Xin-du~2(1.Department of Chemistry,Xi'an University of Arts and Science,Xi'an 710065, China;(2.Institute of Modern Separation Science,Northwest University,Xi'an 710069,China);Refolding of Reduced and Denatured Ribonuclease A by High Performance Hydrophobic Interaction Chromatography[J];Journal of Xi'an University of Arts and Science(Natural Science Edition);2006-02
10 BAIQuan,KONG Yu,ZHANG Yang- jun,GENG Xin- du (Institute of Modern Separation Science,Key L ab of Modern Separation Science in Shannxi Province,Northwest University,Xi′ an710 0 6 9,China);Studieson the refolding of reduced-denatured insulin on a hydrophobic surface[J];;2000-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved