IMMUNOHISTOCHEMICAL AND IMMUNOELECTRONMICROSCOPIC STUDY OF MHC ANTIGENS EXPRESSIONON HEPATOCYTES IN CHRONIC HEPATITIS B
Wang Hong,Yao Guang-bi Cinical Immunological Research Unit Shanghai jin An District Central Hospital.
The intrahepatic MHC antigens expression in 22 patients with serum HBeAg postitivechronic hepatitis B were studied using monoclonal antibodies and ABC immunohistochemicaltechniques. MHC classⅠ(HLA-ABC)antigens were displayed on the plasma membrane ofparenchymal hepatocytes in 2 of 4 CPH patients and in all of 18 CAH patients. The positivehepatocytes were mainly in the areas of piecemeaL necrosis and intralobular focal necrosis.The extent of display of MHC-Ⅰantigens were correlated to the infiltration of CD8-Tlymphocytes and liver cell damage. Immunoelectron microscopy study showed that thepresence of MHC-Ⅰantigens were found on the membrane of hepatocytes and the positivehepatocytes had close contact with lymphocytes which had passed through the sinusoid.Expression of MHC classⅡantigen (DR)on hepatocytes could not be detected in all CPHpatients and were found in 11 of 18 CAH patients. But the DR positive hepatocytes appearedless than the expression of MHC-Ⅰantigens,and were distributed in focal pattern.This studysuggested that the display of MHC antigens may play an important role on the pathogenesisof hepatitis B.