IMMUNOHISTOCHEMICAL AND IMMUNOELECTRONMICROSCOPIC STUDY OF MHC ANTIGENS EXPRESSIONON HEPATOCYTES IN CHRONIC HEPATITIS B
Wang Hong,Yao Guang-bi
Cinical Immunological Research Unit Shanghai jin An District Central Hospital.
The
intrahepatic MHC antigens expression in 22 patients with serum HBeAg postitivechronic
hepatitis B were studied using monoclonal antibodies and ABC
immunohistochemicaltechniques. MHC classⅠ(HLA-ABC)antigens were displayed on the
plasma membrane ofparenchymal hepatocytes in 2 of 4 CPH patients and in all of 18 CAH
patients. The positivehepatocytes were mainly in the areas of piecemeaL necrosis and
intralobular focal necrosis.The extent of display of MHC-Ⅰantigens were correlated to the
infiltration of CD8-Tlymphocytes and liver cell damage. Immunoelectron microscopy study
showed that thepresence of MHC-Ⅰantigens were found on the membrane of hepatocytes and
the positivehepatocytes had close contact with lymphocytes which had passed through the
sinusoid.Expression of MHC classⅡantigen (DR)on hepatocytes could not be detected in all
CPHpatients and were found in 11 of 18 CAH patients. But the DR positive hepatocytes
appearedless than the expression of MHC-Ⅰantigens,and were distributed in focal pattern.This
studysuggested that the display of MHC antigens may play an important role on the
pathogenesisof hepatitis B.
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.