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STUDY ON MECHANIZMS OF SECRETING BICARBONATEBY DUODENAL MUCOSA IN EABBIT

Yao Bi-guang,Hogan DL,Isenberg JI.First Affiliated Hospital,University.  
uodenal surface cells secrete bi carbonate that provides a barrier against injury.Experiments were performed to identify duodenal bicarbonate ragulation and transportpathways.Rabbit proximal duodenal mucosa were mounted in chambers under short-circuitedconditions.Bicarbonate transports,short-circuit current(Isc,and potential difference PD)were quantitated in response to prostaglandin E2(PGE2),vasoactive intestinal polypeptide(VIP),dibutyryl cyclic adenosine monophosphate(db-cAMP) and electrical field stimulation(EFS).Anoxia(N2),4,4'- diisothiocyanostilbene-2,2’-disulfonic acid(DIDS) and Cl-freesolutions,ouabain,and Na+-free solution were also studie,as was the effect of VIP and PGE2on duodenocyte cAMP.PGE2,VIP,db-cAMP and EFS significantly increased bicarbonatesecretion,Isc and PD.Ourabain,Na+-free solutions, anoxia inhibited the responses.DIDS andCl-free solutions abolished the PGE2-induced response,reduced the reponse to VIP by about50%,and had no effect on the response to db-cAMP.Tetrodotoxin(TTX)inhibited the EFSresponse.After PGE2 and VIP,cAMP concentration increased,yet was likely independent ofbicarbonate secretion.
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