THE EFFECT OF TNFαON NIH/3T3 CELL,LIVER LIPOCYTE PROLIFEBATION AND PROCOLLAGEN EXPSSION
Gao Chun-fang,Kong Xian-tao,Fan Lie-ying.second Military Medical University,Changzheng Hospital
NFαwhich was produced mainly by mononuclear cells and macrophages had definiteeffect on fibroblast proliferation,but its effect on collagen production was controversial.Theeffect of recombinant TNFα on NIH/3T3 cell and rat lipocytes proliferation as well as typeⅠand type Ⅲ procollagen gene expression were studied.The results showed that TNFαstimulated the proliferation of NIH/3T3 cell and lipocyte in dose-dependent and inhibited thetypeⅠand type Ⅲ procollagen expression at the level of individual cell. The study revealedthat rat liver lipocyte is comparable with NIH/3T3 cell in the presence of rTNFα. TNFα isone of the important mediators in the fibrosis initiation and regulation.