Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
Add to Favorite Get Latest Update

Experimental study on cross linkage damage of biological molecules in old rats

Guo Yubin;Xu Guixiang;Zhang Kechun,et al.(Department of Biochemistry, Hunan College of Traditional Chinese Medicine,Changsha 410007)  
Objective To explore the relation between aging and the cross linkage damage of biological molecules(DNA, protein).Metbod Old (18 months) and young(5 months) rats of Wistar strain were used and DNA was prepared from the liver of the tats. The melting temperature(Tm)and the hyperchromic effect of DNA were measured. In addition, the thermal contraction temperature, the collagen deposition capacity and the soluble collagen content in caudal tendon were assaied to explore the relation between aging and the cross linkage damage in collagen.Results The Tm(73.2 ±1.1℃)and the hyperchromic effect (1.42±0.08) of hepatic DNA in young rats were lower than those in old rats (74.3±1.0℃,1.45±0.03).the difference of Tm between old and young rats was significant(P0.05).The thermal contraction temperature(67.2±1.6℃) and the collagen deposition capacity(613.6±74.6μg/mg)in caudal tendon of old rats were higher than those of young rats (64.1± 2.3℃,529.7±46.3μg/mg) (P0.01). The soluble collagen content in caudal tendon of young rats (51.5±3.8μg/mg) was a little higher than that of old rats (50.5±3.3μg/mg) (P0.05).Conclusions These results suggest that the cross linkage damage of DNA and collagen increase in old rats.
【CateGory Index】: R339.38
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 ;高龄肺癌病人术前心肺功能不全与术后并发症的护理[J];Journal of Nurses Training;2001-09
2 CHEN Zhao-he, YANG De-zhong (The Institute of Drug of Guangzhou Military Logistics Department, Guangzhou 510500, China);Effect and Harm of Trace Elements on the Old People Health[J];Trace Elements Science;2002-08
3 Li Linghui;The comprehensive nursing for elderly stroke patients with dysphagia[J];Journal of Nurses Training;2011-16
4 Chen Yuanwu (Department of Physical Culture);HUMBLE OPINION ON THE HARMFUL EFFECTS OF STRENUOUS PHYSICAL EXERCISE ON HUMAN BODY'S HEALTH[J];Journal of Hubei University(Natural Science Edition);1993-02
5 ZHANG Gui-min,TANG Wen-jie,BAN Jing,MA Li-xin (School of Life Science,Hubei University,Wuhan 430062,China);An efficient protocol for extraction of fungal chromosomal DNA[J];Journal of Hubei University(Natural Science);2006-01
6 JIN Culling, YING Jie, LIUMin;Application of Breath Training Device in the Elderly During Perioperation[J];Journal of Nursing Science;2004-06
8 Zhang Ming1 Zhai Chaoyang2 Zhao Chengyu1 Wan Qian21Department of Medical Biology and Cell Biology,School of BasicMedical Sciences (Chengdu 610041);  2.Laboratory of Molecular Biology;Properties of Chromosomal DNA of Termitomyces albuminosus[J];JOURNAL OF WEST CHINA UNIVERSITY OF MEDICAL SCIENCES;1997-04
9 Song Qilin et al (Department of Metabolic Disease);Biomarker Expressed in Three dimension Method of Aging[J];JOURNAL OF HARBIN MEDICAL UNIVERSITY;1997-01
10 ZHONG Shi-jun, OU Gang-wei, GE Zheng-long (Department of Biochemistry, Zunyi Medical College, Zunyi 563003, China);Influence of high glucose concentration on the expression of TGF-β gene,excretion of LN and colage-IV in HUVEC[J];Basic Medical Sciences and Clinics;2004-04
Chinese Journal Full-text Database 10 Hits
1 Han Mingxiang et al;Primary Research of Model "Dificiency-Blood Stasis-Senility[J];Journal of Anhui Traditional Chinese Medical College;1992-03
2 Yan Dexin (Shanghai Railway Central Hospital, Shanghai);Deficiency of Qi and Stagnancy of Blood——an Important Mechanism of PhysicaI Senility[J];;1989-02
3 WANG Hong-li ~(1),WU Tie~(2),WU Zhi-hua~(2),CHEN Ken~(1),GUO Qi-jie~(1*) (1.Department of Clinical Medicine,Guangdong Pharmaceutical College,Guangzhou 510305,China;2.Department of Pharmacology,Guangdong Medical College,Zhanjiang 524023);Anti-aging effects of ginsenosides,tanshinone and chuanxionyzine on mice skin: a comparison study[J];Academic Journal of Second Military Medical University;2006-05
4 Yao Xuedong, Lin Qiren, Shi Jinxing, et al (Department of Orthopaedics, The Affiliated 2nd Hospital, Fujian Medical University, Quanzhou, 362000);Experimental Study of Collagenase in the Prevention of Adhesion of Intrathecal Tendon[J];JOURNAL OF FUJIAN MEDICAL UNIVERSITY;1999-02
5 DENG Yue e\+1,JI Li jin\+2\;(1.Dept.of Modern Medicine,FCTCM,Fuzhou,Fujian 350003,China;\;2.Dept.of Traditional Chinese Medicine,FCTCM,Fuzhou,Fujian 350003,China) \;;Study Correlation Between Emotion and Spleen & Stomach[J];Journal of Fujian College of Traditional Chinese Medicine;2003-03
6 WANG Hong li 1, WU Tie 2, WU Zhi hua 2, ZHU Yong xiang 3, GUO Qi jie 1 (1.Department of Dermatology, Guangdong College of Pharmacy, Guangzhou,Guangdong 510224; 2.Department of Pharmacology, Guangdong Medical College, Zhanjiang,Guangdong 524023);Experimental study on anti-aging effect of Ginsenosides on skin of the aging model mice induced by D-galactose[J];Academic Journal of Guangdong College of Pharmacy;2003-01
7 SHI Yu, JIANG Xian, LI Li. Department of Dermatology, Huaxi Hospital of Sichuan University, Chengdu 610041, China;Wrinkles and Skin Photoaging[J];International Journal of Dermatology and Venereology;2006-03
8 Du Qingyun;Ning Wangrong;Zhang Mingqiong;Lin Xiaofang (Department of Pharmacy,the 175th Hospital of PLA,);Study of Turmeric Volatile Oil Decreasing Human Face Skin Roughness[J];STRAIT PHARMACEUTICAL JOURNAL;1999-01
9 LI Yi, WANG Canling Department of Physiology,Nanyang Medical College,Nanyan 473058;Effects of Liuwei Dihuang Tang on skin senility in mice[J];Journal of Zhengzhou University(Science Medical);2005-06
10 ZHAO Lei,WEI Xiaodong,GE Tangdong,LIU Miao,ZHANG Pengxia(The College of Basic Medicine of Jiamusi University,Jiamusi 154007,China);Effects of Astragalus Root serum on senile cell antioxidation and p16 expression[J];Heilongjiang Medicine and Pharmacy;2006-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved