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《CHINESE JOURNAL OF CERIATRICS》 1996-06
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Experimental study on cross linkage damage of biological molecules in old rats

Guo Yubin;Xu Guixiang;Zhang Kechun,et al.(Department of Biochemistry, Hunan College of Traditional Chinese Medicine,Changsha 410007)  
Objective To explore the relation between aging and the cross linkage damage of biological molecules(DNA, protein).Metbod Old (18 months) and young(5 months) rats of Wistar strain were used and DNA was prepared from the liver of the tats. The melting temperature(Tm)and the hyperchromic effect of DNA were measured. In addition, the thermal contraction temperature, the collagen deposition capacity and the soluble collagen content in caudal tendon were assaied to explore the relation between aging and the cross linkage damage in collagen.Results The Tm(73.2 ±1.1℃)and the hyperchromic effect (1.42±0.08) of hepatic DNA in young rats were lower than those in old rats (74.3±1.0℃,1.45±0.03).the difference of Tm between old and young rats was significant(P0.05).The thermal contraction temperature(67.2±1.6℃) and the collagen deposition capacity(613.6±74.6μg/mg)in caudal tendon of old rats were higher than those of young rats (64.1± 2.3℃,529.7±46.3μg/mg) (P0.01). The soluble collagen content in caudal tendon of young rats (51.5±3.8μg/mg) was a little higher than that of old rats (50.5±3.3μg/mg) (P0.05).Conclusions These results suggest that the cross linkage damage of DNA and collagen increase in old rats.
【CateGory Index】: R339.38
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