The stability of human urine microRNA-126 and its diagnosis value for the renal injury
Liu Yang;Liang Hongyan;Gao Guangqiang;Jiang Xiaofeng;Department of Clinical Laboratory, the Fourth Clinical College, Harbin Medical University;
Objective To analyze the stability of microRNA-126(miRNA-126) in human urine and evaluate its possibility in diagnosis of renal injury. Methods The influence of incubation time, test temperature, numbers of freeze-thaw cycles, pH value and different albumin concentration on stability of microRNA-126 in the urine was evaluated by one variable Chi-Square analysis. Real-time PCR was used to compare the levels of urinary miRNA-126 between 69 healthy volunteers and 139 patients with renal injury. T test was used to compare the variance of urinary miRNA-126 levels between the healthy and patient groups. ROC curve was used to evaluate the value of urine miRNA-126 in diagnosis of renal injury. Results The levels of urinary miRNA-126 were 3.81±0.33, 3.78±0.32, 3.83±0.43, 3.69±0.30 when the samples were incubated at room temperature, 4℃,-20℃,-80℃ for 24 hours respectively. The concentration of urinary miRNA-126 were 3.81±0.33, 3.75±0.39, 3.66±0.33, 3.76±0.32, 3.62±0.39 when the samples were placed at room temperature for 0, 3, 6, 12, 24 h. The levels of urinary miRNA-126 were 3.75±0.29, 3.70±0.32, 3.70±0.31, 3.56±0.29 when urinary specimens were subjected to different freeze-thaw cycles(0, 2, 4, 8). The levels of urinary miRNA-126 were 3.76±0.28, 3.72±0.28, 3.65±0.33 when urinary specimens were subjected to different pH values(4.5, 7.0, 9.0). The concentrations of urinary miRNA-126 in groups(0-20, 40-80, 120-140 mg/L) containing different urinary albumins(3.72±0.30, 3.63±0.38, 3.70±0.50) showed no signifi cantly change. There were no signifi cant differences in the levels of miRNA-126(F value of 1.840, 1.321, 2.262, 0.941, 0.411, all of P0.05). The level of urinary miRNA-126 in patients with renal injury(4.55±0.63) was higher than that in healthy volunteers(3.75±0.39, t=6.231, P=0.002). ROC analysis revealed that miRNA-126 had an AUC of 0.779(95%CI: 0.681-0.877, P0.001) and sensitivity of 61.7%, specifi city of 88.9% when the cut-off value was lg2(50-Ct) 4.19. Conclusion These results indicated that miRNA-126 in urine is relatively stable in urine. It has the potential to become a new molecular biomarker for early diagnosis of renal injury.
【CateGory Index】： R692