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《Chinese Journal of Dermatology》 2004-09
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Detecting Serum IgM-antibody in Cured Leprosy Patients by ND-ELISA: A Result of Three-Year Followe up

WU Qin-xue*, ZHANG Liang-fen, CHEN Xiao-hong, YIN Yue-ping, YU Yan-hua, LI Zhi-cheng, YU Hua, SHI Yue-jun, LU Cheng-zhi, LIN Jun-lun, FENG Su-ying, LI Xiao-jie. *Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China  
Objective To investigate the possibility of detecting relapse of leprosy early by immuno-serological method. Methods Indirect enzyme-linked immunosorbent assay(ELISA) using natural disaccharide octyl bovine serum albumin (ND-O-BSA) as an antigen(i.e. ND-IgM-ELISA or ND-ELISA) to detect antibody against ND in the sera from normal controls, and to detect the change of antibody level in the sera from cured leprosy and from relapsed leprosy patients. Results A total of 666 cured leprosy patients treated with DDS monotherapy, including 430 cases of multi-bacillary patients(P-MB), 236 cases of pauci-bacillary patients(P-PB), and 15 of them were relapsed, their IgM antibody levels (IgM-AbL) were periodically determined by using above ND-ELISA. The results showed that after DDS monotherapy: ①In MB patients, 95 were Ab positive, 12 of them were diagnosed as relapsed leprosy; 335 cases were Ab negative, only 1 of them was diagnosed as relapsed leprosy; ②In PB patients, 44 were Ab positive and 192 cases Ab negative; 1 case of relapsed leprosy was found; ③The risk of relapse was 6.7 times higher in MB patients than that in PB patients; ④In the group with positive Ab, the cumulative rate of relapse(CRR) was 13.68%, in the group of negative Ab, the CRR was 0.35%, the risk ratio (RR)=36.7(RR1), attributable risk (AR)=13.33%; ⑤Even though the serum specimens were taken from PB patients, the Ab of majouity of patients converted to positive when relapse occurred. In the majority of patients relapsed the levels of IgM-AbL tended to be increasing or pesistently positive. Usually relapse occurred 1-2 years after IgM-AbL was converted to positive. Relapse occurred 11-30 years after the patients were cured. In very rare case downgrading(from tuberculous to borderline leprosy) occurred. ⑥The levels of IgM-AbL gradually decreased in all relapsed patients after effective treatment except one case whose IgM-AbL was persistently positive. Conclusions The above results indicate that the ND-ELISA might be useful in screening early M.leprae infection and in predicting and monitoring the relapse of leprosy, especially in multibacillary patients.
【Fund】: 国家自然科学基金资助课题(39670680)
【CateGory Index】: R755
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【References】
Chinese Journal Full-text Database 1 Hits
1 LI Cai-xia1, ZOU Zi-hong2, XU Yuan-pin3, ZHOU Xiao-hong4 (1. The Dermatology Department of Yan’an Hospital, Kunming 650051,China; 2. The Kunming Center for Disease Control and Prevention, Kunming 650011, China; 3. Chuxiong Center for Disease Control and Prevention, Chuxiong 675000,China; 4. The Second Affiliated Hospital of Kunming Medical University, Kunming 650032, China);The Level of Th17 and T Regulatory Cells(Treg)in Cured Leprosy People[J];The Chinese Journal of Dermatovenereology;2013-03
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Yin Yaoping et al;Serological Activity of Recombinant α-Antigen of M.Leprae[J];;1997-02
2 YIN Yueping, WU Qinxue, ZHANG Liangfen, et al. Institute of Dermatology, Chinese Academy of Medical Sciences& Peking Union Medical College, Nanjing 210042;Application of Genome Sequencing Analysis for Genotyping of Clinically Isolated Strains of Mycobacterium Leprae[J];Chinese Journal of Dermatology;2000-S1
3 WU Qinxue, YIN Yueping, ZHANG Liangfen, et al. Institute of Dermatology, Chinese Academy of Medical Sciences& Peking Union Medical College, Nanjing 210042;Preliminary Investigation of Genotype and Its Distribution of Mycobacterium Leprae Strains in Part of China[J];Chinese Journal of Dermatology;2000-S1
4 Wu Qinxue, et al (Institute of Dermatology, Nanjing);Ms-ELISA for Detection of Serum Antibody Level in Leprosy Patient——Establishment of Ms-ELISA[J];Acta Academiae Medicinae Sinicae;1988-06
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 Wu Qinxue; Li Xinyu; Huo Wei, at al. (Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing.210042);A Study on evaluation and application of methods for early serodiagnosis of leprosy[J];CHINA JOURNAL OF LEPROSY AND SKIN DISEASES;1999-02
2 Ying Yueping; Wu Qinxue; Hou Wei. (Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, 210042);An applied research on a new recombinant M.leprae antigen: A preliminary study on the role of α 2-ELISA in mouitoring leprosy relapse[J];CHINA JOURNAL OF LEPROSY AND SKIN DISEASES;1999-03
3 Wu Qinxue, Zhang Liangfen, Yin Yueping, et al. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing,210042 [;A comparative study on serological activities and their trends for four antigens in sera from normal controls and cured leprosy patients[J];China Journal of Leprosy and Skin Diseases;2001-04
4 Tian Xiujun,Liu Jian,Wang Zheng,et al.Beijing Tropical Medicine Research Institute,Beijing Friendship Hospital,Capital University of Medical Sciences,Beijing,100050;Preliminary study on strain genotyping of Mycobacterium leprae collected from Yunnan,Sichuan and Guizhou Provinces[J];China Journal of Leprosy and Skin Diseases;2006-12
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 WU Kui1,2,BI Yu-tian1,2,WANG Yao-li1,2,WANG Chang-zheng1(1Institute of Respiratory Disease,Xinqiao Hospital,Chongqing 400037,2Department of Respiratory Disease,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing 400042,China);Changes of phenotype and function of human CD4~+CD25~- T cells induced after Foxp3 transfection[J];Acta Academiae Medicinae Militaris Tertiae;2008-03
2 LIN Xi-ran~1,HUANG Tian~2 (1 The First Affiliated Hospital of Dalian University,Dalian 116011,China;2 The Second Affiliated Hospital of Dalian University,Dalian 116027,China);Th_(17) Cells and Skin Diseases[J];The Chinese Journal of Dermatovenereology;2009-02
3 NIU Xin-wu,CAO Wei,FENG Jie,PENG Zhen-hui,YUAN Jing-yi(Department of Dermatology,Second Hospital of Xi'an Jiaotong University,Xi'an 710004,China);The Effect of Acitretin on Expresion of IL-17 in Lesions of Psoriatic Patients[J];The Chinese Journal of Dermatovenereology;2010-07
4 QIAN Qi-feng, LU Chuang-lin, ZHANG Ming-xia. Shenzhen Institute of Dermatology, Center for STD Control and Research, Shenzhen 518020, China;CD4~+ CD25~+ Foxp3~+ regulatory T cells in peripheral blood of patients with recurrent genital herpes[J];Chinese Journal of Dermatology;2006-12
5 ZHENG Bi-Zhong,GAO Jin-Cheng, YU Hai-Yan et al.Station for Skin Diseases Control, Lanxi, Zhejiang 321100,China;Levels of tumor necrosis factor alpha and soluble interleukin-2 receptor in serum of the cured leprosy persons[J];Chinese Journal of Immunology;2003-08
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Wu Qin-Xue;Preliminary Study on Serological Activity of Phenolic Glycolipid and it's Aoplication in Diagnosis of Leprosy[J];;1985-00
2 WU Qinxue, YING Yueping, ZHANG Liangfen, et al. Institute of Dermatology, Chinese Academy of Medical Sciences& Peking Union Medical College, Nanjing 210042;Gene Amplification Test of Mycobacterium Leprae in Paraffin- Embedded Tissue[J];Chinese Journal of Dermatology;2000-S1
3 Wu Qinxue, Davis GA and Douglas JT(Institute of Dermatology, Nanjing) University of Hawaii,USA;Evaluation of Blocking Agents for ELISA in Detection of Leprosy Antibody[J];Acta Academiae Medicinae Sinicae;1987-03
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