Transplantation of cultured human fetal pituitary cells in nude mice
Li Yan; Zhang Si-jiong; Qian Wei-ping, et al. (Department of Obstetrics and Gynecology, Second Affiliated Hospital, Henan Medical University, Zhengzhou 450003)
To find a simple and effective treatment for Sheehan's Syndrome, we investigated the transplantation of human fetal pituitary in nude mice. Six nude mice were divided into three groups: transplanted with cultured pituitary cells, with pituitary tissue fragments, and with only RPMI 1640 medium. Blood samples were collected periodically and the FSH and LH levels were detected by ELISA method. The differences between all 3 groups were significant. The hormone levels in the cultured pituitary cell group were persistently high in 30 days. The results showed that the cultured pituitary cells could survive in vivo and could secrete hormones when the grafts were transplanted subcutaneously in the neck of the nude mice.