Experimental cryopreservation of human bone marrow cells in Dimethylsulfoxide and Hydroxyethyl Starch
Luo Ke-huan; Wu Guan-qing; Wang Qi-lu, et al. (Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021)
Fractionated human bone marrow cells could be successfully cryopreserved in a DMSO/HES/Albumin mixture prepared by a modified Ringer's solution as well as the conventional cryoprotectant (10% DMSO) with either-80℃ freezer or programmed freezer. However, the toxicity to suppress granulocyte-macrophage progenitors (CFU-GM) of the former was lower than that of the latter at room temperature, and-80℃ freezer with its advantages of simplicity and easy standardization could substitute for rate-contrulled freezer.