Ad-ING4 inhibits K562 cell growth
YU Xin ZHANG Hai-feng WANG Jin-zhi XIE Yu-feng YANG Ji-cheng MIAO Jing-cheng Cell and Molecular Biology Institute,College of Medicine,Soochow University,Suzhou 215123,China
Objective To observe the effect of recombinant adenovirus Ad-ING4 on K562 cells. Methods Human ING4 recombinant transfer vector pAdTrack-CMV-ING4 was constructed by enzyme digest and ligation of human ING4 gene which was obtained through site specific point mutation of mouse ING4.The vector was co-transduced into BJ5183 E.coli with pAdEasy-1.The new recombinant adenovirus vector pAdEasy-1-pAdTrack-CMV-hING4 was transfected into QBI-293A cells.To obtain the ING4 reeombined ade- novirus(Ad-ING4).Ad-ING4 was used to infect K562 cells.The effect on K562 cells of ING4 was tested by LSCM FCM and immunohistochemistry.Results Human ING4 recombinant adenovirus vector was construc- ted successfully,and high titre ING4 recombinant adenovirus(Ad-ING4)was obtained.[NG4 can down-regu- late the expression of bcl-2 and up-regulate expression of bax.The apoptosis of K562 cells induced by ING4 was proved by LSCM FCM and immunohistochemistry.The apoptosis rate was 19.7%(after 72h),which displayed significant difference compared with that of control groups(P0.01).Conclusion Ad-ING4 can inhibit the growth of K562 cells and induce the cells apoptosis,The human ING4 recombinant adenoviral vec- tor constructed might provide an approach to the target therapy of tumors.