Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Effect of Notch ligand Delta-1 on the differentiation and maturation of erythroid progenitors in humans

YU Zhao-cai LIU Wen-chao LIU Du-hu FAN Li Department of Oncology,Xijing Hospital,the Fourth Military Medical University,Xi'an 710032,China  
Objective To explore the biological effect of Notch ligand Delta-1(Notch L 8-1)on the sIL-6R during the differentiation of erythroid hematopoiesis.Methods Mononuclear cells(MNCs)was iso- lated from the normal cord blood using Ficoll graduation solution.MNCs were enriched for CD34~+CD38~- cells by CD34 immunomagnetic beads and a FACS Vantage.CD34~+CD38~-cells was cultured for 7 days in the presence of SCF,Flt3L,TPO and IL-3(4GFs).The cultured cells was detected for the expression of IL- 6R and GPA.The subsequently enriched CD36~+erythroid progenitors were sorted for cells with IL-6R~+and IL-6R~-using FACS Vantage.The CD36~+GPA~-IL-6R~-cells were respectively cultured in the 4GFs、4GFs+ IL-6 or 4GFs+FP6 containing medium in the presence or absence of Notch L δ-1 for 14 days and CD36~+ GPA~(high)red cells were counted.Results IL-6R cells accouted for 95% of CD36~+GPA~+cells.The CD36~+ GPA~-cells was clearly divided into IL-6R~+(46%)and IL-6R~-(54%)subpopulations,the IL-6R~+cell sub- population formed only a few GM colonies(2.1±1.8)and a greater number of BFU-E colonies were genera- ted from the IL-6R~-subpopulation(58.2±18.1)(P0.05).The number of CD36~+GPA~(high)cell was (1.400±0.180)×10~6 in the presence of FP6,lower than that[(2.460±0.190)×10~6]in the presence of FP6+Notch L δ-1(P0.05).Conclusion Notch L δ-1 enhances the sIL-6R-mediated effects of IL-6 on the generation of erythiod cells.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved