Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《CHINESE JOURNAL OF HEMATOLOGY》 1994-01
Add to Favorite Get Latest Update

RT/PCR DETECTION OF AML_1-ETO FUSION GENE INACUTE MYELOGENOUS LEUKEMIA M_(2b)

Zhang Tong; Huan Wei;Tong Jian Hua;et al(Rui Jin Hospital, Shanghai Second Medical University,Shonghai, 200025)  
his paper dealt with the transcriptional expression ofthe AML_1-ETO fusion gene transcripts in acute myelogenous leukemia (AML) M_(2b) phenotype with t (8 ;21).We developed a specific “nested” reverse transcriptase/polymerase chain reaction (RT/PCR). This rapid andsensitive method proved useful not only for the diagnosisof AML-M_(2b) subtype, but also for the detection of theminimal residual disease as it might detect one leukemiccell out of 10 ̄4 ̄ 10 ̄5 normal cells during the clinicalremission. Among the 11 AML-M_(2b) patientsinvestigated , PCR amplification products were obtainedin 10 cases while the RT/PCR was negative in one t(8;21) case, suggested possible molecular heterogenicity.The role that the AML-ETO fusion gene played in theleukemogenesis needs further studies.
【Fund】: 卫生部、国家教委和上海市高教局基金
【CateGory Index】: R733.71
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved