DETECTION OF AML_1-ETO FUSION TRANSCRIPT INACUTE MYELOGENOUS LEUKEMIA WITH POLYMERASECHAIN REACTON TECHNIQUE
Le Xiao feng; Chen Shanshan;Chang KS; et al(Institute of Hemaiology, Beijing Medical University,Beijing ,100034)
e used one set primer (ETOU_1/ETOD_1 ) which wascapable to amplify the AML_1-ETO fusion transcript in17 cases of AML by reverse transcriptase polymerasechain reaction (RT/PCR). A predictable 200-bp DNAfragment could be amplified in all 12 untreated cases (11patients were FAB-M_2, 1 FAB- M_4).of 12cases,typical t(8; 21) or its variants were found in 7 withcytogenetics assay. The size of the amplified DNAfragment and the patterns of restriction digest in allsamples were identical, which indicated that t (8; 21 )translocation breakpoint occured within a single intron ofthe AML_1 and ETO genes. We also found the residualAML_1- ETO fusion mRNA in another 5 FAB-M_2patients who had been in cornplete remission for 2 to 38months. This results showed that minimal residualleukemic cells persisted in the bone marrows of thosepatients. We concluded that detection of AML_1-ETOfusion transcript was a powerful tool for diagnosis andmonitoring of the t (8 ; 21) positive AML.