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STUDY ON THE PRODUCTION OF TUMOR NECROSIS FACTOR (TNF)IN LEUKEMIA CELLS FROM CHILDREN WITH VARIOUS TYPES OF LEUKEMIA

Zhou Yanwen ;Komada Y; Zhang Xiaoli ;et al(Medical Faculty of Mie University ,Japan , 200092)  
he in vitro production of tumor necrosis factor-α(TNF-α) induced by the streptococal preparation OK432was examined to assess the monokine production capaci-ty of the leukernic cells. The TNF-α-producing cells innormal peripheral blood were monocytes when stimulat -ed with OK433 or 12-0-tetradecanoyl-phobol 13-acetate(TAP) , while both lymphocytes and monocytes could be stimulatcd to produce TNF-α with PHA-P.The capacity of the leukemic cells from 43 patientswith acute lymphoblastic leukemia ( ALL) , 13with acute nonlymphocytic leukemia (ANLL) and 2with chronic myelogenous leukemia (CML) to produceTVFα was investigated in the present study. In 43 ALLpatients. only 7 could produce relatively small amount ofTVFα. Among them, 4 were common ALL, 2 undiffer-enuated ALL and 1 B-cell ALL, two of them also ex-pressed myeloid antigens on the same leukemic cells, 2cases with undifferentiated type of ALL under one yearof age. In 18 cases of ANLL, 2 of 3 M_1 and 1 of 3 M_2could produce TVFα, all cases with M_4 ( 6 cases) andM_(5b) (2 cases) subtype revealed large amount of TNFαproduction, while none of M_(5a) (2 cases) secreted TNFαin vitro. Both of CML patients showed srnall arnount ofTNFα production. The capacity to produce TNFα inleukemia cells was wcll correiated with the exnressioii ofmonocyte specific CD14 antigen. so the TVFa produc-tion in childhood leukemia would be one of the cbarac-teristic of leukemic cells with monocytoid differentiation.
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