IN VITRO PURGING OF ACUTE LYMPHOBLASTICLEUKEMIA CELL LINES WITH VINCRISTINE ANDMETHYLPREDNISOLONE
Shi Hong;Yan Wenwei;Han Junling;et al(Institute of Hematology,CAMS,Tianjin,300020)
be purging effects of vincristine(VCR)or methyl-prednisolone(MP)alone and in combination on threeleukemia cell lines(Nalm-6, Duadi,K562 )were stud-ied. The effects of the drugs were analyzed with multi-ple drug-effect analysis method. The results showed thatthe purging effect of the combination of VCR and MPwith various molar ratios was synergistic for the Nalm-6and Daudi cell lines. Compared with VCR or MP alone,the dose of each drug in combination decreased 3.1～ 11. 43 and 3.4～24.96 folds respectively for obtainingthe same killing efficacy,The colonies of the Nalm-6and Daudi cells were declined to undetctale to undetectable level,while the CFU-GM still survived 17.5±3.5% in combi-nation of the two drugs. Our results suggest that thepurging effect of this combination in the autologous bonemarrow transplantation for acute lymphoblastic leukemiapatlents is promising.