MECHANISM OF TOLERANCE INDUCED IN ADULT MICEAND ITS APPLICATION TO ALLOGENEIC BONEMARROW TRANSPLANTATION
Bai Ailin;Xie Shusheng;Long Zhenzhou;et al(Department of Immunology,Beijing Medical Universi-ty,Beijing,100083)
sing intravenous injection of allogeneic spleen cellsplus bone marrow cells followed by intraperitoneal injec-tion of cyclophosphamide and antithymocyte serum,weachieved a long term survival of skin allograft in adultmice. Invivo and in vitro transfer experiments did notshow any suppressive activity in the spleen cells of thetolerant mice, FACS analysis of direct immunofluo-rescence test sbowed that a mixed microcbimerismdevel-oped in the thymus of the tolerant mice,which suggest-ed that the mechanism of the tolerance might be mainlyinvlved in the.clonal deletion,When tolerant mice wereued as donors in the allogeneic bone marrow transplan-tation, the graft versus host disease(GVHD)could becompletely avoided in the recipients. The reconstitute re-cipients established a stable chimerism and could toleratethe growth of donor-derived tumor graft.