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Analysis of clonality with immunoglobulin heavy chain gene rearrangement fingerprinting map in childhood acute lymphoblastic leukemia

Liu Jihua ,Lu Shangen,Zhu Ping,et al.  Department of Pediatrics ,PLA General Hospital,Beijing 100853  
Objective:To investigate the types of the immunoglobulin heavy chain (IgH) gene rearrangement and the co existing clones in childhood acute lymphoblastic leukemia (ALL).Methods:Polymerase chain reaction (PCR) amplification and single strand conformation polymorphism (SSCP) were used to construct the IgH PCR SSCP gene fingerprinting.Results:Of the 34 gene fingerprinting maps obtained from newly diagnosed ALL ,9 (32.14%) were one allelic rearrangement and 15(53.75%) were bi allelic in mono clonal rearrangements.Four cases (14.29%) with high white cell count and precursor B immunophenotype were oligoclonal rearrangements.Conclusion:There were multiple IgH bi allelic rearrangements and co existing clones in childhood ALL.
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