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Preliminary study on the arsenic trioxide induced NB4 cell apoptosis and its molecular mechanisms

Chen Guoqiang *,Zhu Jun,Shi Xuegeng,et al. * Shanghai Institute of Hematology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025  
Objective:To illustrate the possible cellular and molecular mechanisms of arsenic trioxide(As 2 O 3 ) in the treatment of acute promyelocytic leukemia(APL).Methods:APL cell line NB4 was used for in vitro studies.The effect of As 2O 3 on APL was studied by using flow cytometry, DNA electrophoresis, Narthern blotting and Western blotting. Results:As 2O 3 induced NB4 cell apoptosis ,while not inhibiting the growth and survival of two other leukemic cell lines (HL 60 and U937).Furthermore,As 2O 3 effectively down regulated the expression of bcl 2 gene without changing the mRNA levels of other apoptosis associated genes (including p53,c myc,bax and bcl XL).Conclusion: These might be one of the molecular mechanisms of As 2O 3 induced NB4 cell apoptosis.
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