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Effects of arsenic trioxide on the subcellular localization of PML/PML RAR α protein in leukemic cells

Ni Jianhua,Chen Guoqiang,Zhu Jun,et al.Shanghai Institute of Hematology,Ruijin Hospital,Shanghai Second Medical University 200025  
Objective:In order to illustrate the possible roles of PML RAR α protein in arsenic trioxide(As 2O 3) induced NB4 cell apoptosis.Methods: Effects of As 2O 3 on the subcellular localization of PML RARα in NB4 cells were studied.Results:① Anti PML serum staining was reduced and PML granules emerged in the perinuclear cytoplasm in a diffuse pattern in HL 60 cells under As 2O 3 treatment; ② abnormal PML/PML RAR α granules were decreased;③ NB4 cells accumulated anti PML serum staining granules in the cytoplasms were increased and similar accumulation also found in apoptotic cells;and ④ pretreatment with all trans retinoic acid (ATRA) for 24 or 48 hours did not alter the As 2O 3 effects.Conclusion: As 2O 3 induced apoptosis was independent of the retinoic acid signal pathway,and it might be regulated by PML/PML RAR α and/or other related genes.
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