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Analysis on RAPD Fingerprints and Genetic Relationship of Astragalus Hybrid

GAO Xia1,ZHAO Jing-feng2,HE Wei-ping2,MA Jin-xing3,YI Feng-yan1(1.College of Ecology and Environmental Science,Inner Mongolia Agricultural University,Hohhot 010019,China;2.Inner Mongolia Grassland Station,Hohhot 010020,China;3.National Grazing Headquarter,Beijing 100026,China)  
In this study,the genetic relationship between the A.membranaceus Bunge,A.adsurgens Pall.and hybrids were analyzed by using RAPD fingerprints,and coefficient of genetic similarity were also studied.The results indicated that a specific band(P 3-670) and 2 specific bands(P 5-680,P 5-870) could be amplified from the Astragalus hybrid by primer 3 and 5.While the paternal parent(A.membranaceus Bunge) and the maternal parent(A.adsurgens Pall.) had no specific bands.Primer 4 could produce a specific band(about 730 bp) to A.membranaceus Bunge,but the other 5 materials had no specific band.Furthermore Astragalus hybrid had a small genetic similarity to its father,while had a big one to its mother.So the genetic characters of Astragalus hybrid were mostly come from its maternal parent.
【Fund】: 全国牧草种质资源保护项目;; 内蒙古科技创新引导奖励资金项目“内蒙古退化植被恢复技术和可持续经营模式研究与示范3-1(4)”
【CateGory Index】: S567.239
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