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《Seed》 2018-01
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The Genetic Diversity ISSR Study on the Fagopyrum Dibotrys(D.Don)Hara of Guizhou

DENG Rong;WANG Anna;ZHANG Dinghong;XIANG Qinghua;Guizhou Academy of Agricultural Sciences;  
Using ISSR-PCR molecular markers method,the genetic diversity and relationship were analyzed in 11 different regions of Fagopyrum dibotrys(D.Don)Hara.Screened out from 40 primers with11 PCR primers,polymorphic clear and reproducible amplification were obtained 103 stable bands,Among them,75 loci were polymorphic,The percentage of polymorphic loci(PPB)was 72.8%.Nei-Li similarity coefficient method was used to estimate the genetic similarity coefficient(GS)between 11 varieties of Fagopyrum dibotrys(D.Don)Hara,Its GS is 0.48-0.90,Genetic diversity is abundant,The genetic distance between 11 varieties was GD 0.10-0.52,An average of 0.27,Interspecific genetic differences were small.Cluster analysis showed:11 varieties are divided into 2 broad categories,In the first category,Dafang and Suicheng are the first to come together,the relationship between the two is close,But the relationship between Guiyang and other 5 areas fromFagopyrum dibotrys(D.Don)Hara is far,so it is clustered separately;Among the second major categories,Luodian and Dushan firstly come together,And the genetic distance is short,The two are genetically related.
【Fund】: 农业科技成果转化资金项目“优质牧草黔金荞麦1号中试与示范”(2014GB 2F200250)
【CateGory Index】: S517
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