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Optimization of Multiple PCR System for Waxy Gene of Wheats in Guizhou

CAO Ning;SUI Jianshu;XIN Zhihai;CHENG Bin;GAO Xu;DING Yanqing;HE Qingcai;ZHANG Liyi;Insititute of Upland Crops,Guizhou Academy of Agricultural Sciences;College of Life Sciences,Guizhou University;  
In order to establish a multiple PCR reaction system for the simultaneous identification of 3glutinous genes(Wx-A1,Wx-B1 and Wx-D1)in Guizhou wheat varieties(lines)through a single reaction process,3specific molecular markers linked to the wheat glutinous gene(Wx-A1 a,Wx-B1 a and Wx-D1 a),MAG 264,BDFL/BRD and MAG 269,were selected to make the molecular marker detection for three glutinous genes(Wx-A1,Wx-B1 and Wx-D1)in the F3 hybrid of the waxy wheat.The reaction system and conditions of the multiple PCR were optimized in this study.As a result,we had screened part or whole of waxy wheat with waxy gene,which provided a theoretical basis for the breeding of Waxy Wheat in Guizhou.
【Fund】: 贵州省科学技术基金项目“贵州糯质小麦的分子标记辅助育种”(黔科合J字[2014]2104号);; 国家小麦产业技术体系贵阳综合试验站(CARS-3-2-43)
【CateGory Index】: S512.1
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