Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Agriculturae Zhejiangensis》 2014-01
Add to Favorite Get Latest Update

N-terminal domain of VPg encoded by Wheat yellow mosaic virus is involved in the interaction with the nucleolar protein Fibrillarin

BIAN Jing;XIANG Rong;SUN Li-ying;CHEN Jian-ping;College of Chemistry and Life Science,Zhejiang Normal University;State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection/Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences;  
Wheat yellow mosaic virus( WYMV) is a member of the genus Bymovirus in the family Potyviridae. WYMV has two single-stranded positive sense RNA genomes which produce 10 viral proteins. Among those proteins, viral genome-linked protein( VPg),which binds to the 5' end of the viral RNA genome,is predicted to contain a nuclear localization signal( NLS) and nuclear export signal( NES) in the N- and C-terminal portions,respectively. WYMV( Wheat yellow mosaic virus) VPg fused to C-terminus of enhanced green florescent protein( eGFP-VPg) exclusively accumulated in the nucleus of epidermal cells of Nicotiana benthamiana plants. Fibrillarin,a nucleolus resident protein,was introduced into eYFP( n) plasmid. To investigate the regions of the VPg domain binding fibrillarin,a series of VPg mutant and fibrillarin performed BiFC assay. The result display VPg localized and interact with fibrillarin in the nucleus. NLS but not NES is required for interact with fibrillarin.
【Fund】: 农业部小麦产业体系病虫害防控(CARS-3-1-14);; 农业部转基因小麦新品种培育子课题(2011ZX08002-001);; “现代农业生物技术与作物病害防控”——浙江省高校重中之重学科建设;; 浙江省植物病毒与组培产业重点领域创新团队(2009R50032)
【CateGory Index】: S435.121
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved