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《Acta Agriculturae Zhejiangensis》 2014-01
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Knock out deletion and functional analysis of glucose transportation system in Xanthomonas campestris pv. campestris

MA Chen;LIANG Ru-bing;LIU Jian-hua;School of Life Science and Technology,Shanghai Jiao Tong University;  
Three glucose transporters and two membrane-bound glucose dehydrogenases were knocked out in the phytopathogenic bacterium Xanthomonas campestris pv. campestris 8004. The five mutants and Xcc8004 had little difference in their basic metabolisms,such as cell growth curve,extra-cellular polysaccharide production and cellulases activities when cultured in nutrition-rich medium. However,the deletion of sodium / glucose transporter gene( XC_ 2460) influenced the cell growth when glucose was used as carbon source,but its extra-cellular glucose yield in carboxymethyl cellulose degradation test was about 1. 67-fold higher than those of other strains. These results demonstrated the hypothesis that the extracellular glucose concentration from cellulose could be increased significantly by block of glucose influx transport system without affecting basic cell metabolism is feasible.
【Fund】: 国家自然科学基金资助项目(30900010);; 教育部新教师基金项目(20090073120066)
【CateGory Index】: Q78;S432.4
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