Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Agriculturae Zhejiangensis》 2014-01
Add to Favorite Get Latest Update

Knock out deletion and functional analysis of glucose transportation system in Xanthomonas campestris pv. campestris

MA Chen;LIANG Ru-bing;LIU Jian-hua;School of Life Science and Technology,Shanghai Jiao Tong University;  
Three glucose transporters and two membrane-bound glucose dehydrogenases were knocked out in the phytopathogenic bacterium Xanthomonas campestris pv. campestris 8004. The five mutants and Xcc8004 had little difference in their basic metabolisms,such as cell growth curve,extra-cellular polysaccharide production and cellulases activities when cultured in nutrition-rich medium. However,the deletion of sodium / glucose transporter gene( XC_ 2460) influenced the cell growth when glucose was used as carbon source,but its extra-cellular glucose yield in carboxymethyl cellulose degradation test was about 1. 67-fold higher than those of other strains. These results demonstrated the hypothesis that the extracellular glucose concentration from cellulose could be increased significantly by block of glucose influx transport system without affecting basic cell metabolism is feasible.
【Fund】: 国家自然科学基金资助项目(30900010);; 教育部新教师基金项目(20090073120066)
【CateGory Index】: Q78;S432.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Su Yang 1,2 Zhang Zheng 1,2 Yang Qi 1,2 Liu Junliang 1,2 Duan Chengjie 1,2 Feng Jiaxun 1,2 1 College of Life Science and Technology,Guangxi University,Nanning,530005;2 Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization,Nanning,530005;Isolation and Identification of Cellulose-Degrading Fungal Strain A25-2 and Enzymology Characterization of Its Produced Cellulases[J];Genomics and Applied Biology;2010-03
2 QIN Yi-min1,2,TAO Heng1,LIU You-yan1,2,LI Qing-yun1,2(1.School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China;2.Guangxi key laboratory of biorefinery,Nanning 530003,China);Screening of the Cellulase-Enhancing-Protein Producing Strain and Optimization of its Culture Conditions[J];Journal of Chemical Engineering of Chinese Universities;2013-01
3 WANG Min1,2,WANG Jie2(1.Department of Life Science,Hengshui University,Hengshui 053000,Hebei,China; 2.College of Food Science and Technology,Agricultural University of Hebei,Baoding 071001,Hebei,China);Optimization of Production of Lignin Peroxidase by Phanerochaete chrysosporium[J];Hubei Agricultural Sciences;2011-11
4 ZHOU Jin1, YIN Peng2 (1. Life Science Division of Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, China; 2. College of Science, Nanjing Forest University, Nanjing, Jiangsu, 210037, China);Expression and One-step Purification of Recombinant Glucosidase in Pichia pastoris[J];The Chinese Journal of Process Engineering;2009-05
5 HE Jiang1,2,MAO Zhong-gui1,2,ZHANG Qing-hua1,2,ZHANG Jian-hua1,2,TANG Lei1,2,ZHANG Hong-jian1,2(1.Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China;2.School of Biotechnology,Jiangnan University,Wuxi 214122,China);Construction of a Microbial Consortium RXS with High Degradation Ability for Cassava Residues and Studies on Its Fermentative Characteristics[J];Environmental Science;2012-03
6 Wu Shu-bin1 Wei Wei-qi1 Liu Li-guo2(1.State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China; 2.Guangzhou Yourui Bioscience Co.,Ltd.,Guangzhou 510663,Guangdong,China);Effect of Alkaline Sulfite Pretreatment on Enzymatic Saccharification of Eucalyptus[J];Journal of South China University of Technology(Natural Science Edition);2011-11
7 Arthur Redding1 Chen Yuan-cai2 Fu Shi-yu2 Zhan Huai-yu2 JayJ.Cheng1(1.Department of Biological and Agricultural Engineering,North Carolina State University,Raleigh NC 27695-7625,USA;2.State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China);Bioethanol Production and Dilute Acid Pretreatment of Lignocellulosic Materials:a Review[J];Journal of South China University of Technology(Natural Science Edition);2012-03
8 AI Bin-ling,WANG Yi-qiang,CHEN Jie-nan(Institute of Biological and Environmental Science and Technology,Central South University of Forestry and Technology,Changsha 410004,China);Enhancement of Bran to Cellulase Production by Trichoderma reesei Rut C-30[J];Biomass Chemical Engineering;2009-02
9 WEI Wei-qi1,WU Shu-bin1,LIU Li-guo2(1.State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,China; 2.Guangzhou You Rui Bio-technology Limited Company,Guangzhou 510640,China);Effect of the Alkaline Sulfite Pretreatment on the Enzymatic Saccharification of Eucalyptus Wood[J];Chemistry and Industry of Forest Products;2011-06
10 WANG Xiao-juan,LI Pei-pei,YUAN Xu-feng,WANG Xiao-fen,CUI Zun-jun(College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University,Beijing 100193,China);Comparison of four microbial communities with wheat straw-degradation capability at normal temperature[J];Journal of China Agricultural University;2011-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved