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《Acta Agriculturae Zhejiangensis》 2019-01
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Adjuvant effect of E515-A on porcine epidemic diarrhea virus inactivated vaccine

MA Yongqi;XU Wei;WANG Yuemin;HU Songhua;College of Animal Sciences,Zhejiang University;  
E515-A was a white oil containing ginseng stem and leaf saponins (GSLS). An oil emulsion vaccine was obtained by mixing E515-A and water phase containing porcine epidemic diarrhea virus (PEDV) antigen at a low shear force (200 r·min~(-1)). To observe the adjuvant effect of E515-A on inactivated PEDV vaccine,72 ICR mice were randomly divided into 6 groups and subcutaneously immunized twice at a two-weeks interval with the following solution,respectively: (1) physiological saline solution, (2) PEDV antigen + physiological saline solution, (3)PEDV antigen + GSLS, (4) PEDV antigen + white oil adjuvant, (5) PEDV antigen + E515-A, (6) PEDV antigen +aluminum hydroxide (alum). Serum IFN-γ and NK cytotoxicity were determined 24 h after primary immunization;serum specific IgG,IgG1 and IgG2a and splenic lymphocyte proliferation were analyzed after booster immunization.The results indicated that E515-A significantly enhanced the immune response to PEDV vaccine when compared with aluminum adjuvant. E515-A significantly promoted the killing activity of NK cells in immunized mice,increased the production of IFN-γ,enhanced the splenic lymphocytes proliferation to Concanavalin (Con A) and lipopolysaccha-ride (LPS) stimulation,and improved the level of serum IgG and its subclass IgG1 and IgG2a in comparison with the control group. To improve the immunity of pigs,E515-A as an adjuvant deserves further study for its use in swine vaccine.
【Fund】: 国家自然科学基金(31672598)
【CateGory Index】: S852.4
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