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《Acta Agriculturae Zhejiangensis》 2019-01
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Correlation analysis of growth traits and two microsatellite polymorphisms in flanking region of growth hormone gene in topmouth culter (Culter alburnus Basilewsky)

LIU Shili;JIANG Wenping;CHENG Shun;CHI Meili;ZHENG Jianbo;JIA Yongyi;ZHAO Jinliang;GU Zhimin;Key Laboratory of Healthy Freshwater Aquaculture/Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province,Ministry of Agriculture and Rural Affairs,Zhejiang Institute of Freshwater Fisheries;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University;  
The body length and weight of 120 topmouth culter (Culter alburnus Basilewsky) cultured in the same pond were measured. The polymorphism information and correlation with growth traits of two microsatellite loci,CalGH01 and Cal-GH02,located in the flanking regions of the growth hormone (GH) gene were analyzed. The results showed that three Cal-GH01 alleles (404,407 and 410 bp) and six genotypes could be detected. The 407 bp allele was the dominant allele,and 407/407 bp was the dominant genotype. The polymorphic information content of the microsatellite locus was 0. 420,and the heterozygosity was 0. 207 2. A total of six alleles (366,375,378,381,384 and 387 bp) and 11 genotypes were detected for Cal-GH02. The 381 bp allele was the dominant allele,and 381/381 bp was the dominant genotype. The polymorphic information content of the microsatellite locus was 0. 450,and the heterozygosity was 0. 466 1,which made it a moderately polymorphic locus. The associations between the polymorphisms of the two loci and growth traits were analyzed using general linear models. The analysis results for microsatellite locus Cal-GH01 showed that 404/407-type individuals accounted for 13. 3% of the sample size and had the longest body length. The 407/410-type individuals accounted for 5% of the sample weight and were the heaviest. There were some differences in body length and body weight among the Cal-GH01 genotypes; however,the differences were not significant (P 0. 05). For microsatellite locus Cal-GH02,366/381-type individuals accounted for 3. 3% of the number of samples,and their body length and body weight were the highest. Their weight was significantly higher than that of the other genotypes (P 0. 05). 384/387-type individuals accounted for 2. 5% of the sample size,and their body length and weight were the lowest. Their body length was significantly lower than that of other genotypes (P 0. 05). In conclusion,we studied the genetic characteristics of two microsatellite loci in 120 fish sampled from the same pond. The correlation analysis for body length and body weight provided a scientific basis for marker-assisted selection for growth traits. Overall,there were associations between polymorphisms in the GH gene and the growth traits of C. alburnus. Therefore,the two microsatellite loci in GH gene could be used as markers for practical breeding programs for growth traits in C. alburnus.
【Fund】: 浙江省农业新品种选育重大科技专项(2012C12907-7 2016C02055-1);; 浙江省淡水水产研究所开放课题(ZJK201508)
【CateGory Index】: S917.4
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Chinese Journal Full-text Database 4 Hits
1 LU Shuang-Qing1, LIU Feng1, 2, 3, LIU Zhen1, ZHANG Jian-She1, XIE Xin-Min2 (1. Department of Biotechnology and Environment Science, Changsha University, Changsha, 410003; 2. College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128; 3. College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai, 200090);COMPARISON IN CLONING AND SEQUENCE OF GROWTH HORMONE GENE IN THREE SPECIES OF GENUS SINIPERCA[J];海洋与湖沼;2008-04
2 Wang Wenjun1,2 Chen Kefei1 Ren Jun1 Gao Jun1 Ding Nengshui1 Li Lin1 Huang Lusheng1** (1.Jiangxi Provincial Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang 330045 ,China; 2. Food Science and Technological Department, Jiangxi Agricultural University,Nanchang 330045,China);Detection of the Polymorphisms of Growth Hormone Gene in Chinese and European Pig Breeds[J];农业生物技术学报;2003-01
3 Nie Qinghua 1 Zhang Xiquan 1 Yang Guanfu 1 Liang Zhiqing2 (1 Department of Animal Science,South China Agricultural University,Guangdong Guangzhou 510642; 2 Department of Zoology,the University of Hong Kong,Hong Kong);PCR-RFLPs Analysis of Chicken Growth Hormone Gene[J];农业生物技术学报;2001-03
4 Yue Peiqi and Luo Yunlin (Institute of Hydrobiology. the Chinese Academy of Sciences, Wuhan 430072);PRELIMINARY STUDIES ON PHYLOGENY OF SUBFAMILY CULTRINAE (CYPRINIFORMES: CYPRINIDAE)[J];水生生物学报;1996-02
Chinese Journal Full-text Database 10 Hits
1 HE Qi;YANG Hong;ZHANG Yong;WANG Fang-fang;TIAN Qi-pei;WEI Shi-wen;ZHANG Xiong;Huaxi Agriculture Bureau of Guiyang;College of Animal Science,Guizhou University/ Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education/Guizhou Key Laboratory of Animal Genetics,Breeding and Reproduction;Teaching Experiment Farm of Guizhou University;Xingyi Agriculture Bureau;;Expression differences of growth hormone(GH)gene in chickens with different shank length[J];广东农业科学;2015-15
2 DUAN Peng-Xiang;YANG Zhi;TANG Hui-Yuan;XIAO Qiong;GONG Yun;XIAO Tiao-Yi;LIU Xiao-Yan;College of Animal Science and Technology, Hunan Agricultural University;Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences;;STUDY ON AGE, GROWTH, REPRODUCTION AND THE STATUS OF RESOURCE DEVELOPMENT OF CULTER OXYCEPHALOIDES(KREYENBERG ET PAPPENHEIM) IN THE XIAO RIVER[J];水生生物学报;2015-04
3 WANG Yong-Mei;TANG Wen-Qiao;Laboratory of Ichthyology,Shanghai Ocean University,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education;;The Number of Vertebrae from Chinese Species of the Cypriniformes and Its Ecological Adaptation[J];动物学杂志;2014-01
4 GU Yang,TIAN Yu-guang,YUE Min,GU Wei-wang(Center of Laboratory Animal and Institute of Comparative Medicine of Southern Medical University,Guangzhou 510515,China);Analysis of the Growth Hormone Gene Segment in Two Species of Chinese Miniature Pigs by PCR-RFLPs[J];中国比较医学杂志;2012-12
5 LIU Jun,ZHAO Jin-liang,ZHANG Min,DAI Wei(Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306,China);Cloning and expression of full-length cDNA of insulin-like growth factor-Ⅱ in mandarin fish Siniperca chuatsi[J];大连海洋大学学报;2012-06
6 HE Bao-li,JIAO Jian-lin,CHEN Li-ling,LI Bo,LI Jin-tao,WANG Li-mei(Dept.of Experimental Zoology,Kunming Medical University,Kunming Yunnan 650500,China);Studies on the Polymorphism of Growth Hormone Gene in Diannan Small-ear Pig[J];昆明医科大学学报;2012-11
7 DAI Wei ZHAO Jin-Liang MIAO Tian-Tian GUO Jin-Tao Key Laboratory of Freshwater Fishery Germplasm Resources,Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306,China;Cloning and Expression of Two Somatostatin Receptor Genes in Siniperca chuatsi[J];动物学杂志;2012-05
8 SONG Xing-chao,YANG Fu-he,LIU Hui-tao,XU Chao,WEI Hai-jun,XING Xiu-mei(Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction,Ministry of Agriculture,State Key Laboratory of Special Economic Animal Molecular Biology,Institute of Special Animal and Plant Science of CAAS,Changchun 130112,China);Bioinformatics Prediction and Analysis on GH Gene of Cervus elaphus[J];经济动物学报;2012-03
9 DAI Wei,ZHAO Jin-liang*,LIU Jun,XUE Yang(Key Laboratory of Freshwater Fishery Germplasm Resources,Ministry of Agriculture, Shanghai Ocean University,Shanghai 201306,China);Cloning,tissue expression of three somatostatin genes of Siniperca chuatsi and their localizations in brain[J];水产学报;2012-09
10 HUANG Bo1,LIU Ruoyu2**,AI Rong1,TIAN Hairong3 (1.Guizhou Animal Husbandry and Veterinary Research Institute,Guiyang,Guizhou 550005; 2.Guizhou University,Guiyang,Guizhou 550025; 3.Guizhou Animal Disease Control and Prevention Center,Guiyang,Guizhou 550008);SNPs Detection of GH Gene and Its Association with Growth Traits in Guizhou Xiaoxiang Chicken[J];中国家禽;2012-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Jin-Liang ① , WANG Wei-Wei, LI Si-Fa, CAI Wan-Qi Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China;Structure of the Mitochondrial DNA Control Region of the Sinipercine Fishes and Their Phylogenetic Relationship[J];遗传学报;2006-09
2 ZHAO Jin-Liang, LI Si-Fa **,CAI Wan-Qi,WANG Wei-WeiKey Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem, Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China;Phylogenetic relationship of sinipercine fishes in East Asia based on cytochrome b sequences analysis[J];动物学报;2006-04
3 BAO Bao-long,LI Jia-le(College of Aqua-life Science and Technology,Shanghai Fisheries University,Shanghai 200090,China);A review of QTL analysis for important aquaculture species[J];上海水产大学学报;2005-04
4 LU Shuang-qing~1,LIU Shao-jun~2,LIU Hong-yu~3,LIU Zhen~1,LIU Yun~2 (1.Department of Biotechnology & Environment Science,Changsha University,Changsha 410003,China;2.College of Life Science,Hunan Normal University,Changsha 410081,China; 3.Department of Environmental Science & Engineering,Hunan University,Changsha 410082,China);Screening of microsatellite primer and its application to conservation genetics of Monopterus albus[J];水产学报;2005-05
5 ZHANG Jing-Nan~1,SONG Ping~(1,①),HU Jia-Rui~2,MO Sai-Jun~1,PENG Mao-Yu~1,ZHOU Wei~1,ZOU Ji-Xing~3,HU Yin-Chang~4(1.Key Laboratory of the Ministry of Education for Developmental Biology,College of Life Sciences,Wuhan University,Wuhan 430072,China;2.Zhongnan Hospital,Wuhan University,Wuhan 430071,China;3.South China Sea Institute of Oceanology,Chinese Academy of Science,Guangzhou 510301,China;4.Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China)[WT5”F5];Molecular Cloning and Sequence Analysis of Full-Length Growth Hormone cDNAs from Six Important Economic Fishes[J];遗传学报;2005-01
6 GONG Cheng-liang, CAO Guang-li, XUE Ren-yu , ZHANG Chuan-xi ( Life Science College , Suzhou University, Suzhou 215006, China);Sequence and structure of encoding eel growth hormone gene[J];水产学报;2002-04
7 SONG Ping1, HU Yin-chang2, XIANG Zhu1, HU Jia-rui3 and PAN Yun-feng1( 1. College of life Sciences , Wuhan University , Wuhan 430072;2. Pearl River Fishenries Research Institute , Chinese Academy of Fisheries Science , Guangzhou 510380; 3. Zhongnan Hospit;CLONING AND SEQUENCING OF FULL LENGTH cDNA IN SILURUS MERIDIONALIS GROWTH HORMONE[J];水生生物学报;2002-03
8 WANG Wen Jun 1,2 , CHEN Ke Fei 1, REN Jun 1, DING Neng Shui 1, LIN Wan Hua 1, GAO Jun 1, AI Hua Shui 1, HUANG Lu Sheng 1,① (1. Jiangxi Agricultural University Jiangxi Provincial Key Laboratory for Animal Biotechnology, Nanchang 330;Relationship of Growth Hormone (GH2) Genotypes with some Production Performances in Pig[J];遗传学报;2002-02
9 ZHANG Yan LI Ning ZHANG Yuan (1 National Laboratory for Agro-biotechnology China Agricultural University Beijing 100094) (2 College of Animal Science and Technology China Agricultural University Beijing 100094);Molecular Cloning and Analysis of the Partial 5′Flanking Regulatory Region of Chicken Growth Hormone Gene[J];遗传学报;1998-05
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