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《Acta Agriculturae Zhejiangensis》 2019-01
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Research and development of primer and probe for identification of porcine-derived materials inferred from TaqMan PCR

GUO Liang;LI Chundong;XU Weiliang;GUO Yuansheng;Xilingol Vocational College;Xilingol Institute of Bioengineering;Xilingol Food Testing and Risk Assessment Center;  
The objective of this study was to design the primer and probe with intellectual property which can be used to detect porcine-derived materials by means of TaqMan PCR. The results showed that the technique based on the primers and probe was specific for species identification and quantification,and was able to detect 1 pg template.This biotechnological method based on TaqMan PCR was appropriate to identify porcine-derived materials in farm products and foods.
【Fund】: 锡林郭勒职业学院重点科研课题(ZD2017-10);; 锡林郭勒生物工程研究院重点科研课题(2016002)
【CateGory Index】: S828
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