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《Acta Agriculturae Zhejiangensis》 2019-01
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Research and development of primer and probe for identification of porcine-derived materials inferred from TaqMan PCR

GUO Liang;LI Chundong;XU Weiliang;GUO Yuansheng;Xilingol Vocational College;Xilingol Institute of Bioengineering;Xilingol Food Testing and Risk Assessment Center;  
The objective of this study was to design the primer and probe with intellectual property which can be used to detect porcine-derived materials by means of TaqMan PCR. The results showed that the technique based on the primers and probe was specific for species identification and quantification,and was able to detect 1 pg template.This biotechnological method based on TaqMan PCR was appropriate to identify porcine-derived materials in farm products and foods.
【Fund】: 锡林郭勒职业学院重点科研课题(ZD2017-10);; 锡林郭勒生物工程研究院重点科研课题(2016002)
【CateGory Index】: S828
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【Citations】
Chinese Journal Full-text Database 3 Hits
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【Co-citations】
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1 MIAO Li;LI Zhijuan;WANG Shan;ZHANG Xiuping;CHEN Jing;Central Quarantine Laboratory of Henan Entry-Exit Inspection and Quarantine Bureau;Identification Consulting Center of Henan Inspection and Quarantine;;Establish and Application of a Real-Time Fluorescence-Based Polymerase Chain Reaction Assay for Detection of Bovine-Derived Materials in Meat Products[J];肉类研究;2015-09
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3 ;Comparison of microsphere digital PCR and real-time fluorescence PCR for detection mutton-derived and porcine-derived ingredients in mutton products[J];肉类工业;2015-07
4 ;Development and application of real- time fluorescent PCR method for detection of turtle's ingredients in meat products[J];肉类工业;2015-06
5 YU Yan;HE Su-ting;WANG Zi-qiang;DENG Feng;Guangdong Institute for Food and Drug Control;Shanghai Institute for Food and Drug Control;;Comparative study of bovine and porcine derived materials in hydrolysate samples by real-time fluorescence quantitative PCR and general PCR[J];中国生化药物杂志;2015-05
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9 GAO Xiao-li;YANG Xin-ting;WANG Dan;ZHAO Lin-na;CHEN Ji-han;HOU Cai-yun;Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality,College of Food Science and Nutritional Engineering, China Agricultural University;Beijing Municipal Center for Food Safety Monitoring;;Detection of Cat-Derived Components in Foods by Real-Time Polymerase Chain Reaction[J];食品科学;2014-24
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