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《Acta Agriculturae Zhejiangensis》 2019-01
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Cloning and expression analysis of β-carotene hydroxylase gene from kale (Brassica oleracea L. var. acephala)

WANG Yushu;WANG Huan;GUO Yu;ZHOU Minghui;CHEN Lu;CHEN Yang;Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas,College of Life Sciences,Agriculture and Forestry,Qiqihar University;College of Chemistry and Chemical Engineering,Qiqihar University;  
The β-carotene hydroxylase gene from kale (Brassica oleracea L. var. acephala) was cloned by homologybased cloning and RT-PCR strategies,named as Bo BCH (Gen Bank accession number: MH016242). Sequence analysis showed that the full-length of Bo BCH c DNA was 906 bp,which encoded a protein with 301 amino acids,an estimated molecular weight was 33. 8 ku and an isoelectric point was 9. 67. The results of conservative structural domain analysis showed that Bo BCH belonged to the FA_hydroxylase protein superfamily. Phylogenetic tree analysis revealed that the kale had the closest evolutionary relationship with Brassica oleracea. Subcellular localization analysis of TMHMM and Wolf-Psort showed that it might be targeted to the chloroplast with four transmembrane regions. The q RTPCR test results indicated that the highest was leaf,the next was stem,and the lowest level was in root; in the different periods of leaves,the expression level in the ornamental period was high,and the expression level was lower in seedling stage and rosette stage.
【Fund】: 国家自然科学基金(31401908);; 黑龙江省自然科学基金(C2016056);; 黑龙江省普通高等学校青年创新人才培养计划(UNPYSCT-2017155);; 黑龙江省省属高等学校基本科研业务费科研项目(135209313)
【CateGory Index】: S681.9
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