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《Acta Agriculturae Zhejiangensis》 2019-01
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Cloning and sequence analysis of two ARF genes and two Aux/IAA genes in Agapanthus praecox ssp. orientalis

YANG Zhou;LYU Ke;LYU Shan;WANG Junjie;ZHANG Di;Department of Landscape Science and Engineering,School of Agriculture and Biology,Shanghai Jiao Tong University;  
Using the Illumina sequencing platform,transcriptome sequencing of Agapanthus praecox ssp. orientalis callus and embryonic callus treated with different concentrations of exogenous auxin picloram (PIC) were performed.Two ARF genes and two Aux/IAA genes that expressed differentially during the auxin signal transduction pathway were obtained and named as Ap ARF1,Ap ARF2,Ap Aux/IAA2 and Ap Aux/IAA3. To further understand their function in auxin signal transduction during somatic embryogenesis,their full-length c DNA sequences were cloned by RACE technique. The full-length c DNA sequences of Ap ARF1,Ap ARF2,Ap Aux/IAA2 and Ap Aux/IAA3 were 2 343,2 888,1 034 and 821 bp,each contained an open reading frame of 1770,2 349,480 and 549 bp,respectively,which encoded 589,782,159 and 182 amino acids,respectively. Through bioinformatic analysis including protein domain analysis,phylogenetic tree analysis,and protein homology analysis,we concluded that Ap ARF1 and Ap ARF2 proteinshad the typical construction of ARFs,and might be related to plant aging; Ap Aux/IAA2 lacked a completed domainⅣ,Ap Aux/IAA3 lacked a completed domain Ⅱ; however,they may still have the typical function of Aux/IAA family. We constructed gene over-expression vectors with p HB vector and transformed them into wild-type Arabidopsis thaliana. The phenotypic results showed that compared with the wide-type,the over-expression of Ap ARF1 and Ap ARF2 could promote the growth and flowering of transgenic plants,while the over-expression of Ap Aux/IAA2 and Aux/IAA3 showed a depressing influence on the growth of transgenic plants. The transgenic results combined with the sequence analysis proved that Ap ARFs and Ap Aux/IAAs had a regulatory role in auxin signal transduction pathway and plant growth.
【Fund】: 国家自然科学基金(31670693 31300580)
【CateGory Index】: S682.19
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