Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Hybrid Rice》 2004-02
Add to Favorite Get Latest Update

Analysis on the Polymorphism among the Parents of Two-line Hybrid Rice by Microsatellite Markers

WANG Zhong-an(College of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China)  
The microsatellite marker or SSR analysis was conducted, using six two-line hybrid rice parents including (Pei′ai) 64S and five restorer lines as the materials. The results showed that 47 of 60 primer pairs could amplify distinct bands among all parents. Using the Nei′s standard genetic distance D as the index to analyze the polymorphism among parents, it was found that the polymorphic rate between Pei′ai 64S and 9311 was highest at 26.9%, followed by that between Pei′ai 64S and E32 at 25.9% and that between Pei′ai 64S and Teqing was lowest at 17.0%, which indicates that the relationship between Pei′ai 64S and 9311 is farthest while that between Pei′ai 64S and Teqing is nearest. According to the results of the cluster analysis by UPGMA method, the tested six parents were classified into three groups. Group One included Teqing, Shengyou 2 and 219, Group Two contained E32 and 9311, and Group Three had only one parent, i.e., Pei′ai 64S. Therefore, SSR markers can be used to rapidly identify the relationship between parents, so the heterosis of a given F_1 hybrid can be predicted and a good reference can be given to the choice of parents in hybrid rice breeding programs.
【Fund】: 福建省科技攻关项目 (K810 79)
【CateGory Index】: S511
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved