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《Chinese Journal of Antibiotics》 2010-01
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A study on microwave induced screening of Streptomyces 702 by resistance-chemicals mutational label

Tu Xuan,Tu Xiao-rong,Zhang Zhi-ping and Tu Guo-quan(Biological Science & Engineering College,Jiangxi Agricultural University,Nanchang 330045)  
Objective To screen out a Streptomyces 702 strain of high yield of antifungal substance by microwave mutation.Methods Gentamicin-resistance mutational labelling selection model with Streptomyces 702 strain as experiment material and gentamicin as sensitive antibiotic was built to screening high-yield mutants.The Streptomyces 702 spores was treated by microwave in different times.Gentamicin-resistant microwave mutant is the single colony which grew on the PDA culture medium containing lethal concentration gentamicin.The single strain was choosed for primary screening and secondary screening,then determined for its yield of antifungi substance.The biological activitiy was mensurated by USP bioassays.Results The results showed that the gentamycin-resistance mutational frequency of the Streptomyces 702 treated by microwave for 30s was up to 23.13% and the lethal rate was 70.53%.High antifungal substance-producing mutant 20-29-47 was isolated in the screening experiments,and its yield of antifungal substance reached 1478μg/ml.which was 49.9% higher than that produced by its parent strain.Conclusions The Streptomyces 702 strain of high antifungal substance could be obtained by microwave mutation and resistance-chemical mutational labelling selection model.
【Fund】: 江西省科技支撑计划项目(2009BNA07200)
【CateGory Index】: S476.1
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