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The Effects of Different Additions and Hormors on the Degeneration of Azadirachta Indica and its Azadirachtin Content

Lei Guangfu\ Zhu Xiru\ Zhang Yunkai\ Zhang Haibao\ Liu Wei(South China Institute of Hotany,Academia Simica,Guangzhou 510650)  
The effects of different additions and hormors to the degeneration of Azadirachta indica A.Juss and its azadirachtin content in the callus were discussed in this paper.The results showed according to experiments:Sucrose (30 g/L) was fitted for callus growth,but glucose (30 g/L) for Azadirachtin accumulation;Inositol could obviously stimulate both growth rate and Azadirachtin synthesis of callus;Different organic supplements had nutrient regulation in callus culture,but the medium supplemented with coconut mild could only raise Azadirachtin content obviously;As we considered both fectors on the growth rate of callus and its azadirachtin content,the most suitable combintion of hormone should be 2,4 D 2.0+6 BA 0.5.
【Fund】: 华南生物科学与技术研究中心资助课题
【CateGory Index】: Q949.753.105;Q94
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