Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《吉林医学院学报(自然科学版)》 1994-01
Add to Favorite Get Latest Update

Detection of anti-HLA-DR antibody in the serum by blocking immunoenzymatic test of Raji cell

Hu Jing Xin Li Dao,et al Department of Pathophysiology. Jilin Medical College. Jilin  
Using the characteristics of HLA-DR antigen expression of Raji cell that was competitively combined by anti-HLA-DR antibody in detected sera and anti-human HLA-DR McAb,the blocking immunoenzymatic method of Raji cell was established to detect anti-HLA -DR antibody in the serum. The soecificity of methodology, its reproducibility, and the dletermination of experimental conditions were studied and then the contenes of anti-HLA-DR antibody in sera of 72 patients with SLE and 113 normal individuals were studied by this method. The results showed that the method displayed stronger specificity and good reproducibility (C. V = 4. 66%),and litre of the anti body was 1:5-1:40. The positive rate of anti-HLA-DR antibody was 39% in sera of SLE patients and 1. 8% in normal individuals. It is thought that the method possesses the advantages of stronger specificity,good reproducibility,simple operation and lower false positive rate for the blocking immunoenzymatic test of Raji cell to detect anti-HLA -DR antibody in the serum,which is worthy of popularization.
【CateGory Index】: R446.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Li Dao, Yang Ting-Bin, et al Department of Pathophystology, Jilin Medical College, Jilin;Expression rate and intensity of HLA-DR on monocytes of the peripheral blood of SLE patients[J];Chinese Journal of Pathophysiology;1992-06
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 YE Jun,CHEN Ya-Bao,JIANG Jun,LIN Mei,ZHANG Li-Xin,TIAN Feng-Xi.Department of Laboratory Medicine,the Taizhou People's Hospital,Taizhou 225300,China;Expression of HL-DR antigen on peripheral blood lymphocytes and monocytes in patients with active stage of SLE[J];Chinese Journal of Immunology;2008-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Wang lu-nan Li shu-qing (Department of microbiology Jinzhou Medical College,Jinzhou);Detection of anti-dsDNA antibody in SLE patients by enzyme marking staphylococcal protein A (SPA)[J];Chinese Journal of Immunology;1987-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved