The effects of riboflavin on lipid metabolism in rats with hyperlipidemia
GU Qing;ZHOU Peng-hui;ZHANG Jing-shu;HE Ning;LIU Ying-hua;LI Jing;ZHANG Da-long;Tianjin Centers for Disease Control and Prevention;
Objective To study the influence of riboflavin on lipid metabolism in rats with hyperlipidemia and the mechanism.Methods Seventy male rats were randomly divided in two groups. Ten rats were used as normal control group, and sixty rats were fed high-fat diets to establish hyperlipidemia model. Eventually the forty hyperlipidemia rats were randomly divided into model control group and the low, middle, high dose group, 10 rats for each group. The riboflavin dose were 0.677, 3.385 and 6.670 mg/kg respectively. The blank control group was fed with normal diet and the model group was fed with high-fat diet. On the 60 th day,lecithin cholesterol acyl transferase(LCAT) activity of blood was measured, the hepatic pathological morphological changes in hepatic tissue were observed by HE staining, the 10% hepatic homogenate was prepared and the total cholesterol(TC),triglyceride(TG) and 3-hydroxy-methyl-glutaryl coenzyme a reductase(HMGR) in hepatic homogenate were detected. Results The hepatic TC in low riboflavin sub-group was(1.07±0.14) mmol/L which was significantly lower than that [(1.37±0.36) mmol/L]in middle riboflavin sub-group(P0.05). The hepatic TG in low and middle riboflavin sub-groups were(1.73±0.28) and(1.82±0.38) mmol/L which were significantly lower than that [(2.15±0.43) mmol/L] in high riboflavin sub-group(P0.05). The hepatic HMGR level in low riboflavin sub-group was(1.82±0.72) ng/ml which was significantly lower than that [(2.88±0.99) ng/ml] in high riboflavin sub-group(P0.05). The serum LCAT level in intervention group was not different from that in high riboflavin sub-group(P0.05). Conclusion Riboflavin could reduce the hepatic lipid by inhibiting the HMGR activity and influence the lipid metabolism.