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《Scientia Agricultura Sinica》 2005-01
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Studies on the Methods of Detecting PVY~N and PVY~O by RT-PCR

SUN Qi, ZHANG Chun-qing (Agronomy College, Shandong Agricultural University, Taian 271018)  
Potato virus Y(PVY) is one of the important viruses infecting potatoes. It influences the production seriously. The nucleotide sequences of potato virus Y N-strain(PVYN)and O-strain(PVYO) were compared. A pair of homologous primers were designed according to the conserved region of coat protein gene. So the RT-PCR method of detecting PVY was built. Judging by the result of comparison, the 3′primer was designed in conserved region and two 5′primers were designed in two variable region. So a complex RT-PCR method was built which could distinguish PVYN and PVYO. The two methods were very powerful for detecting virus-free seed potatoes, investigating PVY and studying its pathology.
【CateGory Index】: S435.32
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