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《Scientia Agricultura Sinica》 2005-09
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Molecular Characterization of Papaya leaf curl virus Infecting Carica papaya in Guangzhou and Its Biological Test

ZHANG Lu-bin,ZHOU Guo-hui,LI Hua-ping,ZHANG Shu-guang(Laboratory of Plant Virology,South China Agricultural University,Guangzhou 510642)  
A geminivirus isolate GT infecting papaya was detected and obtained from Guangzhou,Guangdong Province.The complete nucleotide sequence of DNA-A of GT was determined to be 2 769 nucleotides in length,and had typical genomic organization of a begomovirus,encoding six ORFs with two ORFs [AV1(CP) and AV2] in virion-sense DNA and four ORFs (AC1-AC4) in complementary-sense DNA.The comparison of complete and intergenic region nucleotide sequences and encoded amino acid sequences for ORFs with other begomoviruses indicated that GT had the highest sequence identity with isolate papaya leaf curl Guangdong virus isolate GD2.The biological test of isolate GT suggested that GT could infect tobacco,tomato and papaya by whiteflies (Bemisia tabaci) transmission.
【Fund】: 广东省“十五”重大科技专项(2002A208010202);; 国家自然科学基金(30370929)资助项目
【CateGory Index】: S436.67
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