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《Scientia Agricultura Sinica》 2005-09
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Molecular Cloning and Sequence Analysis of Genes Encoding GOBP1 and PBP in the Antenna of Helicoverpa assulta(Guenée)

WU Shao-ying1,WANG Gui-rong2,WU Kong-ming2,GUO Yu-yuan2,YUAN Guo-hui1,GUO Xian-ru1(1 College of Plant Protection,Henan Agricultural University,Zhengzhou 450002;2 Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094)  
Genes encoding GOBP1 and PBP in the antenna of Helicoverpa assulta(Guenée)were sequenced.Two pairs of primers were designed based on the comparison of GOBP1 and PBP gene sequences of Helicoverpa armigera reported previously.Two specific bands (about 400 bp in length) were amplified from cDNA of H.assulta antenna.Two segments were cloned into T-easy vector,respectively.Sequencing and structural analysis showed that the full-length of GOBP1 ORF was 441bp,147 amino acid residues were encoded.The predicted MW and PI were 17.2 kD and 4.71,respectively.The full length of PBP ORF is 405bp,135 amino acid residues were encoded.The predicted MW and PI were 15.1 kD and 5.2.The two sequences were deposited in GenBank and the accession number is AY864774 and AY864775.
【Fund】: 河南省自然科学基金资助项目(0311031700)
【CateGory Index】: S433
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