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《Scientia Agricultura Sinica》 2005-09
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Expression of PKCα in Pulmonary Arterioles and Its Association with Pulmonary Vascular Remodeling in Broilers with Pulmonary Hypertension Induced by Cold Temperature Exposure

TAN Xun,LIU Yan-juan,PAN Jia-qiang,LI Jin-chun,SUN Wei-dong,WANG Xiao-long(Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls,Nanjing Agricultural University,Nanjing210095)  
The present study was conducted to examine the expression of protein kinase Cα(PKCα)in pulmonary arterioles in broilers with pulmonary hypertension induced by cold temperature exposure and the relationship between PKCαand pulmonary vascular remodeling.One hundred and sixty Avian-2000broilers were divided equally into a normal temperature control group(NT)and a low temperature group(LT)at14-day-old of age.The group LT was subjected to low temperatures by lowering1-2°C per day from28°C(day14)down to14-12°C(day21),and remained constant until the end of the experiment,while the group NT was still reared at normal temperatures(house temperature was20°C from day21on).PHS incidence was recorded.Ratio of right/total ventricle(RV/TV),packed cell volume(PCV)and hemoglobin(Hb)were measured at24,32,39and45-day-old of age,respectively.Vessel wall area to vessel total area(WA/TA),mean media thickness in pulmonary arterioles(mMTPA)were determined using computer-image analytic software,and expression of PKCαin the pulmonary arterioles labeled by immunohistochemistry and quantified by measuring optical density(OD)using computer-image analytic software.PHS incidence amounted to3.75%and12.5%in groups NT and LT,respectively(P0.05).PCV in the group LT was elevated after day32(P0.05),and RV/TV ratio increased on day45(P0.05).Both the WA/TA and mMTPA of broilers in the group LT were significantly elevated(P0.05).There were no differences in OD value between the two groups before day 32 (P 0.05).However,one week later (at day 39 of age),the OD value of broilers in the group LT was significantly increased (P 0.05).The increased OD values were positively correlated with mMTPA and WA/TA.These results demonstrated that PKCα expression was increased in broilers with pulmonary hypertension,and increased expression of PKCα might enhance pulmonary vascular remodeling.
【Fund】: 国家自然科学基金(30371061);; 江苏省“九五”农业重点攻关项目(BE964888)
【CateGory Index】: S858.31
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