Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Scientia Agricultura Sinica》 2006-01
Add to Favorite Get Latest Update

Protein Expression and Function of the vpr Gene of the vpr Isogenic Knockout E.coli Mutant

SUN Jian-He1,YAN Ya-Xian1,LU Cheng-Ping2(1College of Agriculture,Shanghai Jiaotong University,Shanghai 201101;2Key Laboratory of Animal Disease Diagnostic and Immunology,Ministry of Agriculture,Nanjing Agricultural University,Nanjiang 210095)  
【Objective】In order to confirm the function of vpr gene.【Method】 The complement of the mutant strain MC1061 △vpr was constructed by translating intact vpr into the vpr isogenic knockout E.coli mutant and identified using PCR.The lytic susceptibility of VT2 bacteriophage C43b to parent strain MC1061,mutant strain MC1061△vpr,complemented strain were detected.The cell walls of above three strains were extracted,respectively.The test of phage-adsorption to cell wall,Vpr protein expression and blotting were also carried out.【Result】 One complemented strain,named MC1061-C,was obtained.The complemented strain returned the susceptibility to the lytic infection of VT2 phage with many small plaques,while the mutant was still resistant the phage.Phage-adsorption test within 30 min showed that 94.8% VT phage could adsorb on the cell walls of complemented MC1061-C,while 85.4% VT phage could not adsorb on the cell walls of mutant MC1061△vpr.The specific peptide of 90 000 from parent and complemented strain were displayed by the Western blot using the rabbit anti-Vpr,the His-Vpr was purified by affinity chromatography.The mutant did not display this protein.【Conclusion】All these revealed that the Vpr supported infection of VT2 phage and most likely be the receptor of VT2 phage.
【Fund】: 上海市教育委员会曙光计划资助项目(B2002186);; 国家自然科学基金资助项目(30571384)
【CateGory Index】: S852.61
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Jia-huan et al(College of Agronomy,Jilin Agricultural University,Changchun,Jilin 130118);Enhancement of the Pathogenicity of Pathogen on Soybean and Cause of Tobacco Hypersensitive Response by hrpZ_(Psg12) Gene of Pseudomonas syringae pv. glycinea[J];Journal of Anhui Agricultural Sciences;2011-15
【Citations】
Chinese Journal Full-text Database 4 Hits
1 HUANG Jian jun (Department of Microbiology, School of Basic Medicine, Third Military Medical University, Chongqing , 400038, China );Resistance of bacteria to the infection of bacteriophage[J];Immunological Journal;2002-S1
2 YAN Ya xian 1, 2 ,LU Cheng ping 2 (1.Department of Animal Science, Shanghai Jiaotong University, Shanghai 201101; 2.Key Laboratory of Animal Disease Diagnostic & Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjiang 21;Construction of recombinant plasmid pYYvpr of gene about receptor of VT bacteriophage in E.coli[J];Journal of Shanghai Jiaotong University (Agricultural Science);2002-02
3 Yan Yaxian 1,2 Sun Jianhe 1 Lu Chengping 2 Heather E Allison 3 ( 1College of Agriculture, Shanghai Jiao Tong University, Shanghai 201101,China) ( 2 Key Laboratory of Animal Disease Diagnostic & Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjiang 210095, China) ( 3School of Biological Sciences,Environmental and Molecular Microbiology Group, University of Liverpool, Liverpool, L69 7ZB UK);Identification of a E.coli VT Phage Receptor (vpr) Gene Mutant Strain[J];Acta Microbiologica Sinica;2002-06
4 luhui-jun1, Han wen-yu1, Lei lian-cheng1, Chen wei2(1.Faculty of Animal Science, The Quartermaster University of PLA, Changchun 130062;2.Xingjiang Tarim University of Agriculture Reclaimation, Alar, Xinjiang 843300; China);Phage Lysis of Drug- resistant Escherichia coli[J];Chinese Journal of Veterinary Drug;2002-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhang Jiahuan Gao Jie Xu Qingguo Yuan Meili (Faculty of Agronomy, Jilin Agricultural University, Changchun 130118);IDENTIFICATION OF THE RESISTANCE OF SOYBEAN VARIETIES TO BACTERIAL BLIGHT DISEASE OF SOYBEAN[J];SOYBEAN SCIENCE;2000-02
2 Zhang Shuzhen~1 Xu Pengfei~1 Han Yingpeng~1 Zhang Dayong~1 Zhao Shijun~1Lei Hong~2 Li Wenbin~1(1.Soybean Research Institute of Northeast Agricultural University,Harbin 150030;2.HeilongjiangUniversity,Harbin 150000; 3.Agriculture Technique Extension Center of Bin County 150400);STUDY ON THE RACE IDENTIFICATION OF PSEUDOMONAS SYRINGAE PV. GLYCINEA AND SCREENING OF RESIATANCE RESOURCE OF SOYBEAN[J];Soybean Science;2006-02
3 QI Chun-yan1,2,LIANG Zheng-wei1,3,YANG Fu1,3,WANG Zhi-chun1,3(1.Northeast Institute of Geography and Agricultural Ecology,The Chinese Academy of Sciences,Changchun 130012,China;2.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China;3.Da′an Sodic Land Experiment Station,Da'an 131317,China);The Physiological Response of Rice Alkali-tolerant Mutant ACR78 to Soda Saline-alkaline Stress[J];Acta Agriculturae Boreali-Sinica;2009-01
4 WANG Yi-jun,MIAO Nan,SHI Ya-ting,DENG De-xiang,BIAN Yun-long ( College of Agronomy,Key Laboratory of Ministry of Education for Plant Functional Genomics,Key Laboratory of Jiangsu Province for Crop Genetics and Physiology,Yangzhou University,Yangzhou 225009,China);Genetic Analysis of a Dominant Dwarf Mutant in Maize[J];Acta Agriculturae Boreali-Sinica;2010-05
5 WANG Shuai,SHEN Huo-lin,WU Xiao-xia(Department of Vegetable,Academy of Agronomy and Bio-technology,China Agricultural University,Beijing 100193,China);Studies on Inheritance and Phenotype of the Yellow Mutant of Celery[J];Acta Agriculturae Boreali-Sinica;2010-S2
6 WANG Yu-quan~1,LI Xiang-yu~2,XU Peng-fei~3,LI Wen-bin~3,ZHAO Shi-jun~3,ZHANG Da-yong~3,HAN Ying-peng~3,ZHANG Shu-zhen~(3*)(1.Vocational Education Center of Baoqing County,Baoqing 155600;2.Crop Breeding Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086;3.Soybean Research Institute of Northeast Agricultural University,Harbin 150030);Study on the Isolation and Identification of Pseudomonas syringae pv. Glycinea and Its Distribution in Heilongjiang Province[J];Heilongjiang Agricultural Sciences;2006-06
7 ZHANG Jiahuan, GAO Jie, YUAN Meili, Li Yu(College of Agronomy, Jilin Agricultural University, Changchun, Jilin 130118, China );Studies on Distribution of Physiological Races of Pseudomonas syringae pv.glycinea[J];Journal of Jilin Agricultural University;2003-01
8 SHENG Ji-gui(Zaozhuang University, Zaozhuang 277160, China);Superhigh-yield cultivation technologies of Huang-Huai-Hai Plain Summer Soybean[J];Inner Mongolia Agricultural Science and Technology;2008-03
9 Zhaoyuan Jiang,Xiaowei Zou,Jie Gao,Qingrong Bai,Jiahuan Zhang(College of Agronomy,Jilin Agricultural University,Changchun 130118,China);Cloning and expressing of a harpin-encoding gene from Pseudomonas syringae pv.glycinea[J];Acta Microbiologica Sinica;2009-10
10 LIU Ya-guang,MA Chao,FENG Lei(College of Agriculture,Northeast Agricultural University,Harbin 150030,China);Study on induced-resistances to bacterial blight disease of soybean from chemical application[J];Chinese Journal of Oil Crop Sciences;2008-01
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 YAN Ya xian 1, 2 ,LU Cheng ping 2 (1.Department of Animal Science, Shanghai Jiaotong University, Shanghai 201101; 2.Key Laboratory of Animal Disease Diagnostic & Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjiang 21;Construction of recombinant plasmid pYYvpr of gene about receptor of VT bacteriophage in E.coli[J];Journal of Shanghai Jiaotong University (Agricultural Science);2002-02
2 Sun Jichang, He Xiaoqing, Zhang Shuzhen Jiangx i H yg ientc and Antiepid emic filiation , Nanchang 330046;A DIAGNOSTIC BACTERIOPHAGE FOR ENTEROBACTER CLOACAE[J];Chinese Journal of Microbiology and Immunology;1995-05
3 CHEN Kangchuan *, GUO Weizhi, CHENG Faji, et al. * Fujian Provincial Centers for Disease Control. Fuzhou, Fujian 350001, China;Investigation on E-coli O157 in Fujian, China[J];CHINESE JOURNAL OF PREVENTIVE MEDICINE;2000-03
4 Chen Kangchuan;Isolation of V. fluvialis Bacteriophage from Water and Sea Food[J];Chinese Journal of Zoonoses;1992-04
5 ZENG Ming,Meng Shufang,Wang Weiyuan,Gu Qingwu (National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050);CLONING AND SEQUENCING OF VT2 GENE FROM E.COLI O157:H7[J];CHINESE JOURNAL OF ZOONOSES;2000-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved