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《Scientia Agricultura Sinica》 2006-01
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Pathotyping of Newcastle Disease Virus by SYBR Green I Real-Time RT-PCR

YI Ying1,2,CHEN Ji-ming1,WANG Zhi-liang1,SUN Cheng-ying1,BAO En-dong2(1Animal Quarantine Institute,Ministry of Agriculture,Qingdao 266032;2College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095)  
【Method】The virulence of Newcastale disease virus(NDV)strains was determined by the lysis site sequences of the viral F protein.Accordingly,two pairs of primers were designed to match such sequences of virulent NDV strains and avirulent strains,respectively.Viral RNA was amplified using the two primers respectively by one-step SYBR Green I RT-PCR,and the virus was pathotypied with the amplification effects of the two pairs of primers and the Tm values of the amplification products.【Conclusion】Pathotyping results of 8 unknown strains of this assay were as the same as those of Mean Death Time(MDT)and sequencing,which suggested that this assay could be used to pathotype NDV strains.
【Fund】: 国家“十五”攻关项目(2002BA514A-18)
【CateGory Index】: S852.65
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【References】
Chinese Journal Full-text Database 3 Hits
1 ZHU Hong-yun;TIAN Guang-lou;College of Agriculture and Animal Husbandry,Tibet University;;Cloning and Sequence Analysis of HN Gene of Newcastle Disease Virus Tibet Isolates[J];中国畜牧兽医;2013-10
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【Co-references】
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1 YU Yang1,2,CUI Jia-ying2,DING Qing-wei2,CUI Peng-fei1,LI Yin2,DENG Guo-hua2,REN Tao1,CHEN Hua-lan2 (1.South China Agricultural University,Guangzhou 510642,China;2.Animal Influenza Laboratory of the Ministry of Agriculture/State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China);Preparation Monoclonal Antibodies of HN Protein Using Newcastle Disease Virus[J];中国畜牧兽医;2011-09
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【Secondary References】
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1 DENG Xian-wen,XIE Zhi-xun,XIE Zhi-qin,LIU Jia-bo,PANG Yao-shan,XIE Li-ji,FAN Qing,LUO Si-si (Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Animal Vaccines and New Technology,Nanning 530001,China);Development of a loop-mediated isothermal amplification assay for visual detection of Mycoplasma iowae[J];中国兽医科学;2013-08
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