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《Scientia Agricultura Sinica》 2013-06
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Effect of FSH on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell

ZHANG Jiao-jiao1,WANG Yi1,ZHANG Hui-qiong1,SUN Si1,SUN Yan1,2,WANG Xian-zhong1,ZHANG Jia-hua1(1College of Animal Science,Veterinary and Aquaculture,Southwest University/Chongqing Key Laboratory of Forage and Herbivorce,Chongqing 400716;2Chongqing Animal Disease Prevention and Control Center,Chongqing 401147)  
【Objective】 The objective of the study is to identify whether or how FSH regulates the mRNA expression of cyclin D1 and cyclin E1 in the cultured immature boar Sertoli cell.【Method】 In the study,the cultured immature boar Sertoli cells were used as materials,and real-time PCR was used to detect the mRNA expression of cyclin D1 and cyclin E1.【Result】 Different doses of FSH(0-100 ng?mL-1) increased the mRNA expression of cyclin D1 and cyclin E1(P0.05),which were peaking at 50 ng?mL-1(P0.05).In addition,FSH(50 ng?mL-1) increased the mRNA expression of cyclin D1 and cyclin E1 in a time-dependent manner(P0.05) and peaked at 30 min(P0.05).Different doses of Forskolin(0-20 μmol?L-1) increased the mRNA expression of cyclin D1 and cyclin E1(P0.05),but lower compared with FSH alone.Rp-cAMP(0-40 μmol?L-1),H-89(0-30 μmol?L-1) and Verapamil(0-100 μg?mL-1) inhibited FSH induced the mRNA expression of cyclin D1 and cyclin E1 in a dose-dependent manner(P0.05),and the combination of Rp-cAMP and Verapamil had more inhibition on FSH activity than individual effect(P0.05),but lower than addition of Rp-cAMP and Verapamil.Furthermore,different doses of U0126(0-10 μmol?L-1) reduced FSH induced the mRNA expression of cyclin D1 and cyclin E1(P0.05),while Rp-cAMP,H-89,Verapamil or U0126 alone had no effect on the mRNA expression of cyclin D1 and cyclin E1 as compared to the control(P0.05).【Conclusion】 FSH regulates the mRNA expression of cyclin D1 and cyclin E1 in a dose-and time-dependent manner,while cAMP-PKA,Ca2+ and extracellular signal-regulated kinase(ERK1/2) is involved in the regulation of FSH on the mRNA expression of cyclin D1 and cyclin E1.
【Fund】: 国家自然科学基金项目(31072183、31101872);; 西南大学研究生科技创新基金项目(ky2011005)
【CateGory Index】: S828
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【References】
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1 Zhang Gui-xue;Li Yu-long;Zeng Yue;Huang He;Zheng Peng;Tian Ya-guang;College of Animal Sciences and Technology, Northeast Agricultural University;;CDC25B Involved in Proliferation of Sertoli Cells of New Born Calves Through FSH and Possibly Being Key Regulating Factor[J];Journal of Northeast Agricultural University(English Edition);2016-01
【Citations】
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1 GAN Rui,ZUO Jing,ZHANG Guo-sheng,ZHU Feng-wei,SUN Yan, WANG Xian-zhong,ZHANG Jia-hua(Chongqing Key Laboratory of Forage & Herbivorous Livestock, College of Animal Science and Technology,Southwest University,Chongqing 400716,China);The Effect of FSH on the Expression of skp2 in Cultured Immature Boar Sertoli Cells[J];畜牧兽医学报;2011-06
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1 DONG Rui-na;DAI Hong;Inner Mongolia Medical University;;Research Progress on Male Reproductive Toxicity Induced by Di(2-ethylhexyl) phthalate[J];世界最新医学信息文摘;2016-90
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5 ZHANG Hui-qiong;SUN Si;ZHANG Jiao-jiao;WANG Yi;WANG Xian-zhong;ZHANG Jia-hua;Chongqing Key Laboratory of Forage & Herbivorce,College of Animal Science,Southwest University;;The effect of FSH on the expression of CyclinA2 in Cultured Immature Boar Sertoli cells[J];中国兽医杂志;2014-09
6 SUN Si;ZHANG Hui-Qing;WANG Yi;ZHANG Jiao-jiao;WANG Xian-zhong;ZHANG Jia-hua;Chongqing Key Laboratory of Forage & Herbivorce, College of Animal Science, Veterinary and Aquaculture,Southwest University;;Effect of FSH on Cultured Immature Boar Sertoli Cell Cycle[J];中国畜牧杂志;2014-05
7 WANGYi,ZHANG Jiao-jiao,WANG Yong,ZHANG Hui-qiong,SUN Si, WANG Xian-zhong*,ZHANG Jia-hua(Chongqing Key Laboratory of Forage & Herbivore,College of Animal Science and Technology,Southwest University,Chongqing 400716,China);Effect of Combination of FSH and 17β-estradiol on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell[J];畜牧兽医学报;2013-06
8 ZHANG Jiao-jiao1,WANG Yi1,ZHANG Hui-qiong1,SUN Si1,SUN Yan1,2,WANG Xian-zhong1,ZHANG Jia-hua1(1College of Animal Science,Veterinary and Aquaculture,Southwest University/Chongqing Key Laboratory of Forage and Herbivorce,Chongqing 400716;2Chongqing Animal Disease Prevention and Control Center,Chongqing 401147);Effect of FSH on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell[J];中国农业科学;2013-06
9 SUN Yan1,2,WANG Xian-zhong1,2,WU Jian-yun1,2,ZHU Feng-wei1, XIANG Yang1,YANG Wei-rong1,ZHANG Jia-hua1,2*(1.Chongqing Key Laboratory of Forage & Herbivore,Southwest University,Chongqing 400716,China;2.College of Animal Science and Technology,Southwest University, Chongqing 400716,China);The Signaling Mechanism of GDNF Expression in Sertoli Cells of Piglet Testis Regulated by FSH[J];畜牧兽医学报;2011-10
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【Co-references】
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1 Li Yu-long;Wu Qiong;Zhao Xun-wu;Zeng Yue;Elkanah Adegoke;Zhang Gui-xue;College of Animal Science and Technology, Northeast Agricultural University;Harbin Pharmaceutical Group Biological Engineering Co., Ltd.;;Influence of FSH Treatment on Expression of CDC25A, TSSK3 and P53 in Vitro Cultured Sertoli Cells of Calf[J];Journal of Northeast Agricultural University(English Edition);2015-01
2 YU Na;YANG Zeyu;LIN Xu;LI Yulong;ZHAO Xunwu;ZHANG Guixue;School of Animal Science and Technology, Northeast Agricultural University;School of Chengdong, Northeast Agricultural University;;Bovine sertoli cells and fibroblast cells culture in vitro and their transfection with the plasmid pEGFP-N3[J];东北农业大学学报;2014-09
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