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《Scientia Agricultura Sinica》 2013-06
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Preparation and Characterization of Ractopamine-Imprinted Material Using Surface-Molecular Imprinting Method and Its Adsorption Characteristics

YANG Ting1,WU Yin-liang1,LI Cun2,ZHAO Jian1,CHEN Guo1,Lü Yan1(1Ningbo Academy of Agricultural Science,Ningbo 315040,Zhejiang;2Tianjin Agriculture University,Tianjin 300384)  
【Objective】 The objective of this experiment is to prepare a molecule-imprinted material on surface of silica gel for ractopamine.【Method】 Polymethylacrylic acid(PMAA) was grafted onto the surface of silica gel particles via the coupling effect of γ-methacryloxypropyl trimethoxy silane,and the grafting particle PMAA/SiO2 was prepared.Then,the molecular imprinting towards the grafted PMAA was performed with ractopamine as template molecule and ethylene glycol diglycidylether(EGDE) as crosslinking agent by using the surface-molecular imprinting technique established,and the ractopamine molecule-imprinted material MIP-PMAA/SiO2 was prepared.The morphology of MIP-PMAA/SiO2 was observed by SEM,and the chemical structure of MIP-PMAA/SiO2 was characterized by FT-IR.Static method was adopted to study the binding properties and molecule recognition characters of MIP-PMAA/SiO2 for ractopamine.【Result】The MIP-PMAA/SiO2 on the adsorption ability of ractopamine was significantly better than clenbuterol and salbutamol.The adsorption capacity of the MIP-PMAA/SiO2 was 11.08 mg?g-1,the specificity binding capacity was 9.93 mg?g-1,and the imprinted factor of the polymer was 9.63.The adsorption reaction reached equilibrium after 10 min,and the desorption ration of ractopamine reached 92.3% in 5 min.【Conclusion】The experimental results show that MIP-PMAA/SiO2 have specific recognition selectivity,excellent binding affinity and elution property for ractopamine.
【Fund】: 国家自然科学基金项目(31072172);; 宁波市农业与社会发展项目(2010C10008)
【CateGory Index】: S859.84
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