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The Genetic Classification of Cytoplasmic Male Sterility Systems in Brassica napus L.

Yang Guangsheng 1 Fu Tingdong 1 Gregory G.Brown 2 ( 1Department of Agronomy, Huazhong Agricultural University, Wuhan 430070; 2Department of Biology, McGill University, 1205 Doctor Penfield, Montreal, Quebec, H3A 1B1 Canada)  
The test on the restoring and maintaining relationship between 9 different cytoplasmic male sterile (CMS) lines showed that Xin 4 2A,Xin 4 3A,Xin 4 4A,Ning 4A, AB1 and pol CMS line Xin 4 1A,S45A and nap CMS line 5021A, have the same restorer and maintainer, respectively. The RFLP and mt DNA fragment polymorphism analysis, cut with four restriction endonucleases of EcoRⅠ, HindⅢ,PstⅠ and SalⅠ, respectively, showed that the cytoplasm of Xin 4 2A,Xin 4 3A,Xin 4 4A,Ning 4A and AB1 is the same as that of Xin 4 1A of pol CMS, and the cytoplasm of S45A is the same as that of Westar S, which has the cytoplasm of nap CMS. Therefore, we may conclude that the Xin 4 1A,Xin 4 2A,Xin 4 3A,Xin 4 4A,Ning 4A and AB1 are pol CMS lines, S45A, 5021A and Westar S are nap CMS lines and P24A is an ogu CMS line.
【Fund】: 国家攀登计划基金
【CateGory Index】: S634.303
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