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《Journal of Fishery Sciences of China》 2007-04
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Development and characterization of a continuous embryonic cell line from southern flounder(Paralichthys lethostigma)

REN Guo-cheng1,CHEN Song-lin2,SHA Zhen-xia2(1.College of Marine Life Science,China Ocean University,Qingdao 266003,China;2.Yellow Sea Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Qingdao 266071,China)  
A continuous cell line,SFEC(southern flounder embryonic cell line),was established from embryos at gastrula stage of a marine cultured fish,southern flounder(Paralichthys lethostigma) and was cultured for more than 240d with more than 80 generation.The SFEC cells were cultured in DMEM medium supplemented with antibiotics,fetal bovine serum(FBS),sea perch serum(SPS),basic fibroblast growth factor(bFGF).The cells were small and round in shape and grew actively and stably in medium.Effects of temperature,FBS and bFGF concentration on the growth of SFEC cells were evaluated.The cells grew well in the temperature range of 24-30℃,but had a reduced growth rate at temperature below 18℃.The growth of SFEC cells in medium containing 15% FBS was higher than that in medium containing 7.5% FBS.Addition of bFGF to the medium significantly increased growth rate of SFEC cells.Chromosome analysis revealed that SFEC cells have a normal diploid karyotype with 2n=6st+42t.GFP reporter gene was successfully transferred into SFEC cells and expressed.
【Fund】: 国家自然科学基金(30170740);; 国家863基础研究与发展(2004AA626110)
【CateGory Index】: S917.4
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