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《JOURNAL OF FISHERY SCIENCES OF CHINA》 1999-01
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A study of isoenzyme phenotypic change in diseased shrimp Penaeus chinensis

Huang Canhua Chen Dihua (Wuhan Institute of Virology,CAS, 430071)  
CIE technique and nucleic acid probe test were used to select diseased shrimps (Penaeus chinensis)which had been infected with viruses, and polyacrylamid gel electrophroresis method was used to study the isoenzyme phenotypic change in diseased shrimps.The bands in the gel were scanned and analyzed through spectrophotometer.There are 4 kinds of isoenzyme (MDH,EST,GOT and SOD) in diseased shrimps.Their patterns are quite different from those in healthy shrimps that phenotypic changes of MDH,EST,GOT reflect the disordered metabolism of carbohydrate,lipid,and protein in diseased shrimps respectively.The weakened activity of SOD indicates that the immunizing function is apparently beginning to fail.Due to the steadiness and good replication of their phenotypic changes,the shrimp isoenzyme can be used as biochemical indices for test and diagnosis.
【CateGory Index】: S945.29
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