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《Chinese Journal of Practical Pediatrics》 2008-01
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Measuring HO-1 in cord blood of full term SGA and its expression in cordVessels

RONG Xiao-ping,SHAO Qin,GUO Xiu-xia,et al.Department of pediatrics,Hebei medical University Forth hospital,Shijiazhuang 050011,China  
Objective To explore the relation of HO-CO system to cause of SGA,searching for a new way in etiology prophylaxis and treatment for it.Methods 50 full term SGA neonates (nosymmetry) in our hospital and obstetric hospital were chosen as objects,that were grouped into two,one named SGA1 was 30 whose mother without pregnancy induced hypertation (PIH),the other named SGA2 was those whose mother with middle or severe PIH.Meanwhile,a group of 20 healthy full term AGA neonates named AGA were chosen as reference.The umbilical artery blood specimens were collected immediately after the newborns were delivered,umbilical cord specimens near placenta were kept as well.HO-1 activity in cord blood was measured by Dual Wavelength Spectrophotometer.HbCO percentage in plasma of cord blood was measured by Chalmers hemoglobin method.The expression of HO-1 in cord blood vessels was detected by the method of immunohistochemistry.Results 1.The comparisons of HO-1 activity and HbCO percentage in the cord blood of different groups of neonates:SGA1 and SGA2 are significantly lower than AGA,shown on statistics with significant difference (P0.01).No significant difference shows between SGA1 and SGA2 (P0.05).2.HO-1activity in cord blood of AGA,SGA1 and SGA2 all shows positive correlation with birth weight (P0.05).3 Expression of HO-1 in cord blood vessels:The intensity of HO-1 expression of both SGA1 and SGA2 are higher than AGA,which shows significant difference (P0.01).No significant difference is found between SGA1 and SGA2 (P0.05).Conclusion HO-1 activity in cord blood correlates closely with intrauterine growth and nutrition of the newborn.
【Fund】: 河北省卫生厅资助项目(2004JB3092-04)
【CateGory Index】: R722
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